Browse

Expansion of human NK cells using K562 cells expressing OX40 ligand and short exposure to IL-21

DC Field Value Language
dc.contributor.authorKweonl, SoonHo-
dc.contributor.authorPhan, Minh-Trang Thi-
dc.contributor.authorChun, Sejong-
dc.contributor.authorYu, HongBi-
dc.contributor.authorKim, Jinho-
dc.contributor.authorKim, Seokho-
dc.contributor.authorLee, Jaemin-
dc.contributor.authorAli, Alaa Kassim-
dc.contributor.authorLee, Seung-Hwan-
dc.contributor.authorKim, Sang-Ki-
dc.contributor.authorDoh, Junsang-
dc.contributor.authorCho, Duck-
dc.date.accessioned2022-06-24T07:15:17Z-
dc.date.available2022-06-24T07:15:17Z-
dc.date.created2020-03-25-
dc.date.issued2019-04-
dc.identifier.citationFrontiers in Immunology, Vol.10 No.APR, p. 879-
dc.identifier.issn1664-3224-
dc.identifier.urihttps://hdl.handle.net/10371/184025-
dc.description.abstractBackground: Natural Killer (NK) cell-based immunotherapy used to treat cancer requires the adoptive transfer of a large number of activated NK cells. Here, we report a new effective method to expand human NK cells ex vivo using K562 cells genetically engineered (GE) to express OX40 ligand (K562-OX40L) in combination with a short exposure to soluble IL-21. In addition, we describe a possible mechanism of the NK cell expansion through the OX40 receptor-OX40 ligand axis which is dependent on NK cell homotypic interaction. Methods: K562-OX40L cells were generated by lentiviral transduction and were used as feeder cells to expand and activate NK cells from PBMCs in the presence of IL-2/IL-15. Soluble IL-21 was also added in various concentrations only once at the beginning of the culture. NK cells were expanded for 4-5 weeks, and the purity, expansion rate, phenotype and function (cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC), cytokine production, CD107a degranulation) of these expanded NK cells were compared to those generated by using K562 feeder cells. Results: The culture of NK cells with K562-OX40L cells in combination with the transient exposure to IL-21 highly enhanced NK cell expansion to approximately 2,000-fold after 4 weeks of culture, compared to a 303-fold expansion using the conventional K562 cells. Mechanistically, the OX40-OX40L axis between the feeder cells and NK cells as well as the homotypic interaction between NK cells through the OX40-OX40L axis were both necessary for NK cell expansion. The short exposure of NK cells to IL-21 had a synergistic effect with OX40 signaling for NK cell expansion. Apart from their enhanced expansion, NK cells grown with K562-OX40L feeder cells were similar to those grown with conventional K562 cells in regard to the surface expression of various receptors, cytotoxicity, ADCC, cytokine secretion, and CD107 degranulation. Conclusion: Our data suggest that OX40 ligand is a potent co-stimulant for the robust expansion of human NK cells and the homotypic NK cell interactions through the OX40-OX40L axis is a mechanism of NK cell expansion.-
dc.language영어-
dc.publisherFrontiers Media S.A.-
dc.titleExpansion of human NK cells using K562 cells expressing OX40 ligand and short exposure to IL-21-
dc.typeArticle-
dc.citation.journaltitleFrontiers in Immunology-
dc.identifier.wosid000465396200002-
dc.identifier.scopusid2-s2.0-85066944479-
dc.citation.numberAPR-
dc.citation.startpage879-
dc.citation.volume10-
dc.identifier.rimsid93627-
dc.description.isOpenAccessY-
dc.contributor.affiliatedAuthorDoh, Junsang-
dc.type.rimsART-
dc.type.docTypeArticle-
dc.description.journalClass1-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Materials Science and Engineering (재료공학부)Journal Papers (저널논문_재료공학부)
Files in This Item:
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse