Publications
Detailed Information
BMP4 signaling plays critical roles in self-renewal of R2i mouse embryonic stem cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Taleahmad, Sara | - |
dc.contributor.author | Salari, Ali | - |
dc.contributor.author | Samadian, Azam | - |
dc.contributor.author | Chae, Se Hyun | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Lee, Bonghee | - |
dc.contributor.author | Bayarsaikhan, Delger | - |
dc.contributor.author | Bayarsaikhan, Govigerel | - |
dc.contributor.author | Lee, Jaesuk | - |
dc.contributor.author | Park, Ji Hwan | - |
dc.contributor.author | Hassani, Seyedeh-Nafiseh | - |
dc.contributor.author | Baharvand, Hossein | - |
dc.contributor.author | Salekdeh, Ghasem Hosseini | - |
dc.date.accessioned | 2022-08-22T09:09:56Z | - |
dc.date.available | 2022-08-22T09:09:56Z | - |
dc.date.created | 2022-06-27 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, Vol.617, pp.8-15 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://hdl.handle.net/10371/184334 | - |
dc.description.abstract | Mouse embryonic stem cells (mESCs) can be maintained in a pluripotent state under R2i culture conditions that inhibit the TGF-I3 and ERK signaling pathways. BMP4 is another member of the TGF-I3 family that plays a crucial role in maintaining the pluripotency state of mESCs. It has been reported that inhibition of BMP4 caused the death of R2i-grown cells. In this study, we used the loss-of-function approach to investigate the role of BMP4 signaling in mESC self-renewal. Inhibition of this pathway with Noggin and dorsomorphin, two bone morphogenetic protein (BMP) antagonists, elicited a quick death of the R2i-grown cells. We showed that the canonical pathway of BMP4 (BMP/SMAD) was dispensable for self-renewal and maintaining pluripotency of these cells. Transcriptome analysis of the BMPi-treated cells revealed that the p53 signaling and two adhesion (AD) and apoptotic mitochondrial change (MT) pathways could be involved in the cell death of the BMPi-treated cells. According to our results, inhibition of BMP4 signaling caused a decrease in cell adhesion and ECM detachment, which triggered anoikis in the R2i-grown cells. Altogether, these findings demonstrate that endogenous BMP signaling is required for the survival of mESCs under the R2i condition.(c) 2022 Published by Elsevier Inc. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | BMP4 signaling plays critical roles in self-renewal of R2i mouse embryonic stem cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.bbrc.2022.05.036 | - |
dc.citation.journaltitle | Biochemical and Biophysical Research Communications | - |
dc.identifier.wosid | 000809804300002 | - |
dc.identifier.scopusid | 2-s2.0-85131443458 | - |
dc.citation.endpage | 15 | - |
dc.citation.startpage | 8 | - |
dc.citation.volume | 617 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Hwang, Daehee | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.