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Effect of Human Peripheral Blood Mononuclear Cells on Mouse Endometrial Cell Proliferation: A Potential Therapeutics for Endometrial Regeneration
DC Field | Value | Language |
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dc.contributor.author | Ahn, Ji Yeon | - |
dc.contributor.author | Hong, Yeon Hee | - |
dc.contributor.author | Kim, Keun Cheon | - |
dc.contributor.author | Kim, Ji Hyang | - |
dc.contributor.author | Lee, Seo-Yeon | - |
dc.contributor.author | Lee, Jung Ryeol | - |
dc.contributor.author | Lee, Eun Ju | - |
dc.date.accessioned | 2022-09-29T03:19:37Z | - |
dc.date.available | 2022-09-29T03:19:37Z | - |
dc.date.created | 2022-07-19 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.citation | Gynecologic and Obstetric Investigation, Vol.87 No.2, pp.105-115 | - |
dc.identifier.issn | 0378-7346 | - |
dc.identifier.uri | https://hdl.handle.net/10371/184734 | - |
dc.description.abstract | © 2022 Objectives: The persistently thin endometrium is a major cause of repeated implantation failure; however, there is no definite treatment for it yet. This study aimed to confirm the potential of human peripheral blood mononuclear cells (hPBMCs) as a therapeutic agent for endometrial regeneration. Design: An experimental study was carried out. Participants/Materials, Setting, Methods: To assess the in vitro effect of hPBMC, the human primary endometrial epithelial cell lines SNU-685 and SNU-1077 were co-cultured with or without 1 × 105 hPBMCs for 24 h. To evaluate the in vivo effect, either 1 × 105 hPBMCs in PBS or PBS alone were injected into the left uterine horn of nonobese diabetic-severe combined immune-deficient mice, and the right untreated uterine horn was used as control. Results: Co-culture with hPBMCs stimulated significant proliferation in both SNU-685 and SNU-1077 cell lines (p = 0.002 and 0.044, respectively). Moreover, treatment with hPBMCs significantly increased the thickness in all parts of the endometrium compared with that in the untreated control uterine horn (proximal: 1.69 ± 0.19 vs. 1.00 ± 0.10, p = 0.009; middle: 1.51 ± 0.14 vs. 1.00 ± 0.12, p = 0.010; distal: 1.72 ± 0.22 vs. 1.00 ± 0.12, p = 0.003, respectively). Compared with the PBS injection group, the hPBMC injection group had significantly thickened endometrium in the middle (p = 0.036) and distal segments (p = 0.002) of the uterine horn. Immunohistochemical analysis revealed the presence of exogenously injected hPBMCs in the uterus of recipient mice. hPBMC-recipient mice had cyclic uterus with normal histology in the endometrium. Limitations: hPBMCs were not applied directly to a mouse model with thin endometrium, so further study is needed. Conclusion: The beneficial effect of hPBMCs on endometrium may suggest their clinical feasibility for the safe treatment of infertile patients with persistently thin endometrium. | - |
dc.language | 영어 | - |
dc.publisher | S. Karger AG | - |
dc.title | Effect of Human Peripheral Blood Mononuclear Cells on Mouse Endometrial Cell Proliferation: A Potential Therapeutics for Endometrial Regeneration | - |
dc.type | Article | - |
dc.identifier.doi | 10.1159/000524232 | - |
dc.citation.journaltitle | Gynecologic and Obstetric Investigation | - |
dc.identifier.scopusid | 2-s2.0-85132455116 | - |
dc.citation.endpage | 115 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 105 | - |
dc.citation.volume | 87 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Jung Ryeol | - |
dc.contributor.affiliatedAuthor | Lee, Eun Ju | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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