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Anticancer activity of IRAK-4 inhibitors against canine lymphoid malignancies
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jun-Hyeong | - |
dc.contributor.author | Lee, Jeong-Hwa | - |
dc.contributor.author | An, Ju-Hyun | - |
dc.contributor.author | Park, Chong-Woo | - |
dc.contributor.author | Choi, Yoon-Pyo | - |
dc.contributor.author | Seo, Seong-Wook | - |
dc.contributor.author | Lee, Hyuk-Woo | - |
dc.contributor.author | Youn, Hwa-Young | - |
dc.date.accessioned | 2022-09-30T05:58:05Z | - |
dc.date.available | 2022-09-30T05:58:05Z | - |
dc.date.created | 2022-08-19 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.citation | Veterinary and comparative oncology, Vol.20 No.3, pp.632-640 | - |
dc.identifier.issn | 1476-5829 | - |
dc.identifier.uri | https://hdl.handle.net/10371/185042 | - |
dc.description.abstract | The interleukin-1 receptor-related kinase 4 (IRAK4), downstream of myd88, plays an essential role in hyperactive TLR signalling seen in some B-cell lymphomas. In particular, efficient IRAK4 inhibitors of activated B-cell subtype of human diffuse large B-Cell lymphoma (DLBCL) are being developed. However, the anticancer effect of IRAK-4 inhibitors in veterinary medicine has not been elucidated. It is therefore explored in this study involving the GL-1 and CL-1 canine lymphoma cell lines in vitro. MyD88 expression was analysed using polymerase chain reaction. GL-1 and CL-1 cells were subjected to concentration- and time-dependent treatment with an IRAK-4 inhibitor and assessed for viability, TLR signalling association and apoptosis using a cell counting Kit-8 assay, Western blotting and flow cytometry. The GL-1 and CL-1 cells exhibited enhanced MyD88 expression, however, canine peripheral blood mononuclear cells (cPBMCs) did not. The IRAK-4 inhibitor reduced cell viability in a dose- and time-dependent manner, significantly reduced the phosphorylation of molecules associated with TLR signalling at IC50 such as IRAK1, IRAK4, NF-kappa B and STAT3, and induced apoptosis in GL-1 and CL-1 cells. The anticancer effect of the IRAK-4 inhibitor on canine lymphoma cells is mediated by apoptosis via downregulation of TLR signalling. | - |
dc.language | 영어 | - |
dc.publisher | Blackwell Publishing Inc. | - |
dc.title | Anticancer activity of IRAK-4 inhibitors against canine lymphoid malignancies | - |
dc.type | Article | - |
dc.identifier.doi | 10.1111/vco.12816 | - |
dc.citation.journaltitle | Veterinary and comparative oncology | - |
dc.identifier.wosid | 000797245600001 | - |
dc.identifier.scopusid | 2-s2.0-85130210496 | - |
dc.citation.endpage | 640 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 632 | - |
dc.citation.volume | 20 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Youn, Hwa-Young | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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