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Elucidating the characteristics of Mx1 and resistance to influenza A virus subtype H1N1 in the newly developed KWM/Hym mice
DC Field | Value | Language |
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dc.contributor.author | Nam, Hajin | - |
dc.contributor.author | Kim, Boyoung | - |
dc.contributor.author | Gautam, Avishekh | - |
dc.contributor.author | Kim, Yoo Y. | - |
dc.contributor.author | Park, Eun S. | - |
dc.contributor.author | Lee, Jong S. | - |
dc.contributor.author | Kwon, Hyung-Joo | - |
dc.contributor.author | Seong, Je K. | - |
dc.contributor.author | Suh, Jun G. | - |
dc.date.accessioned | 2022-09-30T05:59:56Z | - |
dc.date.available | 2022-09-30T05:59:56Z | - |
dc.date.issued | 2022-09-08 | - |
dc.identifier.citation | Laboratory Animal Research. 2022 Sep 08;38(1):28 | - |
dc.identifier.uri | https://doi.org/10.1186/s42826-022-00138-z | - |
dc.identifier.uri | https://hdl.handle.net/10371/185111 | - |
dc.description.abstract | Abstract
Background Inbred mice have several advantages, including genetic similarity to humans, a well-established gene manipulation system, and strong tolerance to inbreeding. However, inbred mice derived from a limited genetic pool have a small genetic diversity. Thus, the development of new inbred strains from wild mice is needed to overcome this limitation. Hence, in this study, we used a new strain of inbred mice called KWM/Hym. We sequenced the Mx1 gene to elucidate the genetic diversities of KWM/Hym mice and observed the biological alterations of the Mx1 protein upon influenza A infection. Results The Mx1 gene in KWM/Hym mice had 2, 4, and 38 nucleotide substitutions compared to those in the Mx1 gene in A2G, CAST/EiJ, and Mus spretus mice, respectively. Moreover, the Mx1 protein in KWM/Hym mice had 2 and 25 amino acid substitutions compared to those in the Mx1 protein in CAST/EiJ and M. spretus mice, respectively. To elucidate the function of the Mx1 protein, we inoculated the influenza A virus (A/WSN/1933) in KWM/Hym mice. Nine days after infection, all infected KWM/Hym mice survived without any weight loss. Four days after infection, the lungs of the infected KWM/Hym mice showed mild alveolitis and loss of bronchiolar epithelium; however, the pulmonary viral titers of the infected KWM/Hym mice were significantly lower than that in the infected BALB/c mice (2.17 × plaque-forming units mL−1). Conclusions Our results demonstrate that the KWM/Hym mice are resistant to influenza A virus infection. Further, these mice can be used as a model organism to understand the mechanism of influenza A virus susceptibility. | - |
dc.title | Elucidating the characteristics of Mx1 and resistance to influenza A virus subtype H1N1 in the newly developed KWM/Hym mice | - |
dc.type | Journal Article | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2022-09-11T03:13:24Z | - |
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