Publications
Detailed Information
A novel nickel-containing superoxide dismutase from Streptomyces spp.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Youn, HD | - |
dc.contributor.author | Kim, EJ | - |
dc.contributor.author | Roe, JH | - |
dc.contributor.author | Hah, YC | - |
dc.contributor.author | Kang, SO | - |
dc.date.accessioned | 2022-10-14T06:06:26Z | - |
dc.date.available | 2022-10-14T06:06:26Z | - |
dc.date.created | 2022-09-05 | - |
dc.date.issued | 1996-09 | - |
dc.identifier.citation | Biochemical Journal, Vol.318, pp.889-896 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | https://hdl.handle.net/10371/186035 | - |
dc.description.abstract | A novel type of superoxide dismutase (SOD) was purified to apparent homogeneity from the cytosolic fractions of Streptomyces sp. IMSNU-1 and Strep. coelicolor ATCC 10147 respectively. Both enzymes were composed of four identical subunits of 13.4 kDa, were stable at pH 4.0-8.0 and up to 70 degrees C, and were inhibited by cyanide and H2O2 but little inhibited by azide. The atomic absorption analyses revealed that both enzymes contain 0.74 g-atom of nickel per mol of subunit. Both enzymes were different from iron-containing SOD and manganese-containing SOD from Escherichia coli, and copper- and zinc-containing SODs from Saccharomyces cerevisiae and bovine erythrocytes, with respect to amino acid composition, N-terminal amino acid sequence and cross-reactivity against antibody. The absorption spectra of both enzymes were identical, exhibiting maxima at 276 and 378 nm, and a broad peak at 531 nm. The EPR spectra of both enzymes were almost identical with that of Nim in a tetragonal symmetry of Ni-III-oligopeptides especially containing histidine. The apoenzymes, lacking in nickel, had no ability to mediate the conversion of superoxide anion radical to hydrogen peroxide, strongly indicating that Ni-III plays a main role in these enzymes. | - |
dc.language | 영어 | - |
dc.publisher | Portland Press, Ltd. | - |
dc.title | A novel nickel-containing superoxide dismutase from Streptomyces spp. | - |
dc.type | Article | - |
dc.identifier.doi | 10.1042/bj3180889 | - |
dc.citation.journaltitle | Biochemical Journal | - |
dc.identifier.wosid | A1996VJ91700022 | - |
dc.identifier.scopusid | 2-s2.0-0029778889 | - |
dc.citation.endpage | 896 | - |
dc.citation.startpage | 889 | - |
dc.citation.volume | 318 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kang, SO | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.