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Enzymatic synthesis of puerarin glucosides using Leuconostoc Dextransucrase
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, Jin-A | - |
dc.contributor.author | Ryu, Young Bae | - |
dc.contributor.author | Park, Tae-Soon | - |
dc.contributor.author | Jeong, Hyung Jae | - |
dc.contributor.author | Kim, Jang-Hoon | - |
dc.contributor.author | Park, Su-Jin | - |
dc.contributor.author | Kim, Joong-Su | - |
dc.contributor.author | Kim, Doman | - |
dc.contributor.author | Kim, Young-Min | - |
dc.contributor.author | Lee, Woo Song | - |
dc.date.accessioned | 2022-10-18T00:38:52Z | - |
dc.date.available | 2022-10-18T00:38:52Z | - |
dc.date.created | 2022-08-31 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.citation | Journal of Microbiology and Biotechnology, Vol.22 No.9, pp.1224-1229 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://hdl.handle.net/10371/186430 | - |
dc.description.abstract | Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials. © The Korean Society for Microbiology and Biotechnology. | - |
dc.language | 영어 | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Enzymatic synthesis of puerarin glucosides using Leuconostoc Dextransucrase | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.1202.02007 | - |
dc.citation.journaltitle | Journal of Microbiology and Biotechnology | - |
dc.identifier.wosid | 000309305400008 | - |
dc.identifier.scopusid | 2-s2.0-84864092589 | - |
dc.citation.endpage | 1229 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 1224 | - |
dc.citation.volume | 22 | - |
dc.identifier.kciid | ART001696415 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Doman | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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