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Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, Jin-A | - |
dc.contributor.author | Jeong, Hyung Jae | - |
dc.contributor.author | Ryu, Young Bae | - |
dc.contributor.author | Park, Su-Jin | - |
dc.contributor.author | Wee, Young-Jung | - |
dc.contributor.author | Kim, Doman | - |
dc.contributor.author | Kim, Young-Min | - |
dc.contributor.author | Lee, Woo Song | - |
dc.date.accessioned | 2022-10-18T00:38:52Z | - |
dc.date.available | 2022-10-18T00:38:52Z | - |
dc.date.created | 2022-08-31 | - |
dc.date.issued | 2012-04 | - |
dc.identifier.citation | Journal of Microbiology and Biotechnology, Vol.22 No.4, pp.510-515 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://hdl.handle.net/10371/186431 | - |
dc.description.abstract | A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD 600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17°C. These results may effectively apply to the heterologous expression of other large DexT genes. © The Korean Society for Microbiology and Biotechnology. | - |
dc.language | 영어 | - |
dc.publisher | 한국미생물·생명공학회 | - |
dc.title | Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system | - |
dc.type | Article | - |
dc.identifier.doi | 10.4014/jmb.1111.11032 | - |
dc.citation.journaltitle | Journal of Microbiology and Biotechnology | - |
dc.identifier.wosid | 000303374000010 | - |
dc.identifier.scopusid | 2-s2.0-84860177427 | - |
dc.citation.endpage | 515 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 510 | - |
dc.citation.volume | 22 | - |
dc.identifier.kciid | ART001654109 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Doman | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
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