Publications

Detailed Information

Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infection

Cited 8 time in Web of Science Cited 8 time in Scopus
Authors

Kang, Taewon; Haque, Mamunul; Lee, Boran; Hong, Kyung Tae; Hong, Seong Cheol; Kim, Younghun; Lee, Jesang; Lee, Jun-Seok; Lee, Dongwhan

Issue Date
2022-10
Publisher
Nature Publishing Group
Citation
Nature Communications, Vol.13 No.1, p. 5841
Abstract
Methods to detect and distinguish the early stage of viral infection often involve complicated and time-consuming protocols. Here, the authors disclose a class of fluorescent molecules that enable fast detection of avian influenza virus infection by selectively localizing at the endoplasmic reticulum in the cell. Cell-based assays can monitor virus infection at a single-cell level with high sensitivity and cost-efficiency. For this purpose, it is crucial to develop molecular probes that respond selectively to physiological changes in live cells. We report stimuli-responsive light-emitters built on a T-shaped benzimidazole platform, and consecutive borylation reactions to produce a library of homologs displaying systematic changes in fluorescence quantum yield and environmental sensitivity. We find that certain fluorophores localize selectively at the endoplasmic reticulum, and interact with proteins involved in the stress signaling pathways. Notably, the mono-borylated compound responds selectively to the stress conditions by enhancing fluorescence, and detects avian influenza virus infection at the single-cell level. Our findings demonstrate the unprecedented practical utility of the stress-responsive molecular probes to differentiate cellular states for early diagnosis.
ISSN
2041-1723
URI
https://hdl.handle.net/10371/186659
DOI
https://doi.org/10.1038/s41467-022-33586-1
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share