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Novel Online Three-Dimensional Separation Expands the Detectable Functional Landscape of Cellular Phosphoproteome
DC Field | Value | Language |
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dc.contributor.author | Kang, Chaewon | - |
dc.contributor.author | Huh, Sunghyun | - |
dc.contributor.author | Nam, Dowoon | - |
dc.contributor.author | Kim, Hokeun | - |
dc.contributor.author | Hong, Jiwon | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Lee, Sang-Won | - |
dc.date.accessioned | 2022-11-23T00:43:24Z | - |
dc.date.available | 2022-11-23T00:43:24Z | - |
dc.date.created | 2022-09-27 | - |
dc.date.issued | 2022-09 | - |
dc.identifier.citation | Analytical Chemistry, Vol.94 No.35, pp.12185-12195 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://hdl.handle.net/10371/187210 | - |
dc.description.abstract | Protein phosphorylation is a prevalent post-translational modification that regulates essentially every aspect of cellular processes. Currently, liquid chromatography-tandem mass spectrometry (LC-MS/MS) with an extensive offline sample fractionation and a phosphopeptide enrichment method is a best practice for deep phosphoproteome profiling, but balancing throughput and profiling depth remains a practical challenge. We present an online three-dimensional separation method for ultradeep phosphoproteome profiling that combines an online two-dimensional liquid chromatography separation and an additional gas-phase separation. This method identified over 100,000 phosphopeptides (>60,000 phosphosites) in HeLa cells during 1.5 days of data acquisition, and the largest HeLa cell phosphoproteome significantly expanded the detectable functional landscape of cellular phosphoproteome. | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | Novel Online Three-Dimensional Separation Expands the Detectable Functional Landscape of Cellular Phosphoproteome | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.analchem.2c02641 | - |
dc.citation.journaltitle | Analytical Chemistry | - |
dc.identifier.wosid | 000848107600001 | - |
dc.identifier.scopusid | 2-s2.0-85137217936 | - |
dc.citation.endpage | 12195 | - |
dc.citation.number | 35 | - |
dc.citation.startpage | 12185 | - |
dc.citation.volume | 94 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Hwang, Daehee | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
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