Publications
Detailed Information
Molecular subgrouping of medulloblastoma in pediatric population using the NanoString assay and comparison with immunohistochemistry methods
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Joo Whan | - |
dc.contributor.author | Park, Sung-Hye | - |
dc.contributor.author | Choi, Seung Ah | - |
dc.contributor.author | Kim, Seung-Ki | - |
dc.contributor.author | Koh, Eun Jung | - |
dc.contributor.author | Won, Jae-Kyung | - |
dc.contributor.author | Nam, Sun Mo | - |
dc.contributor.author | Phi, Ji Hoon | - |
dc.date.accessioned | 2022-12-27T02:13:42Z | - |
dc.date.available | 2022-12-27T11:14:40Z | - |
dc.date.issued | 2022-11-28 | - |
dc.identifier.citation | BMC Cancer, 22(1):1221 | ko_KR |
dc.identifier.issn | 1471-2407 | - |
dc.identifier.uri | https://doi.org/10.1186/s12885-022-10328-6 | - |
dc.identifier.uri | https://hdl.handle.net/10371/187356 | - |
dc.description.abstract | Purpose
Molecular subgrouping of medulloblastoma has become important due to its impact on risk group stratification. Immunohistochemistry (IHC) has been widely used but it has innate limitations. The NanoString assay has been proposed as an alternative method. This study aims to present the characteristics of medulloblastoma subgrouped by the NanoString assay and to compare the subgrouping results with the IHC method. Methods Pediatric patients with histological diagnosis of medulloblastoma who underwent surgery from 2007 to 2021 were included. Clinical characteristics, pathological findings were reviewed. Molecular subgrouping was performed by IHC and by NanoString nCounter Elements TagSets assay. Test for concordance between two methods was made. Results Among a total of 101 patients analyzed, subgrouping using the NanoString assay resulted in 14 (13.8%) WNT, 20 (19.8%) SHH, 18 (17.8%) Group 3, and 39 (38.6%) Group 4 subgroup cases. Survival analysis revealed the following from best to worse prognosis: WNT, Group 4, SHH, and Group 3. In SHH subgroup the large cell/anaplastic histology was present in 30% of cases. Seventy-one cases were analyzed for concordance between NanoString and IHC. Cohens kappa value indicated moderate agreement but identification of Groups 3 and 4 with IHC using NPR3 and KCNA1 markers exhibited poor results. Conclusions The NanoString assay of Korean medulloblastoma patients revealed a more aggressive clinical course in the SHH subgroup which may be explained by a higher proportion of large cell/anaplastic histology being present in this subgroup. IHC did not distinguish Group 3 or 4 accurately. The NanoString assay may represent a good alternative method for practical use in the clinical field. | ko_KR |
dc.description.sponsorship | This study was jointly supported by a grant from the Seoul National University Hospital (No. 0420183140; to Phi JH) and a grant from the National Research Foundation (NRF) of Korea (No. 2018R1D1A1A02086005; to Phi JH) | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Medulloblastoma | - |
dc.subject | Molecular | - |
dc.subject | Subgroup | - |
dc.subject | Method | - |
dc.subject | Immunohistochemistry | - |
dc.title | Molecular subgrouping of medulloblastoma in pediatric population using the NanoString assay and comparison with immunohistochemistry methods | ko_KR |
dc.type | Article | ko_KR |
dc.identifier.doi | 10.1186/s12885-022-10328-6 | ko_KR |
dc.citation.journaltitle | BMC Cancer | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2022-12-04T04:12:34Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.startpage | 1221 | ko_KR |
dc.citation.volume | 22 | ko_KR |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.