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Fat Graft with Allograft Adipose Matrix and Magnesium Hydroxide-Incorporated PLGA Microspheres for Effective Soft Tissue Reconstruction : 효과적 연부조직 재건을 위한 동종지방기질 및 수산화마그네슘 함유 폴리(락틱-코-글리콜산) 미립구를 이용한 지방이식술
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 이정찬 | - |
dc.contributor.author | 김대희 | - |
dc.date.accessioned | 2022-12-29T08:54:37Z | - |
dc.date.available | 2022-12-29T08:54:37Z | - |
dc.date.issued | 2022 | - |
dc.identifier.other | 000000172516 | - |
dc.identifier.uri | https://hdl.handle.net/10371/188360 | - |
dc.identifier.uri | https://dcollection.snu.ac.kr/common/orgView/000000172516 | ko_KR |
dc.description | 학위논문(박사) -- 서울대학교대학원 : 의과대학 의학과, 2022. 8. 이정찬. | - |
dc.description.abstract | Autologous fat grafting is one of the most common procedures used in plastic surgery to correct soft tissue deficiency or depression deformity. However, its clinical outcomes are often suboptimal, and lack of metabolic and architectural support at recipient sites affects fat graft survival leading to complications such as cyst formation and calcification. Extracellular matrix (ECM)-based scaffolds, such as allograft adipose matrix (AAM) and poly (lactic-co-glycolic acid) (PLGA), have shown exceptional clinical promise as regenerative scaffolds. AAM is composed of the extracellular matrices of adipose tissue through decellularization. Additionally, PLGA is one of the most commonly used biopolymers owing to its biodegradability for tissue regenerations. Magnesium hydroxide (MH), an alkaline ceramic, has attracted attention as a potential additive to improve biocompatibility. Magnesium hydroxide (MH) could neutralize the acidic microenvironment induced by the acidic decomposed products of PLGA, thereby suppressing undesirable inflammatory reactions. We attempted to combine fat graft with injectable regenerative scaffolds: natural (AAM) and synthetic (PLGA/MH microspheres). We investigated the volume changes and viability of injected fat graft in relation to the effects of biomaterials. In this study, a comparison of the volume retention effect and angiogenic ability in vivo between autologous fat grafting, injectable natural (AAM), and synthetic (PLGA/MH microsphere) biomaterials will provide a reasonable basis for fat grafting. And by using PLGA/MH microsphere, we could overcome the disadvantages of conventional PLGA scaffolds and effectively improve fat retention rate with complex biological functions. | - |
dc.description.tableofcontents | 1. Introduction 1
2. Materials and Methods 5 Materials 5 Fabrication of the PLGA microspheres 5 Scaffold characterization and in vitro degradation test 6 Cell cytotoxicity assay 6 In vitro wound healing assay 7 Quantitative real-time PCR 7 Fat preparation 8 Allograft adipose matrix preparation 8 Animal study 8 Histological analysis 9 Statistical analysis 10 3. Results and Discussion 10 Scaffold fabrication and characterization 10 In vitro biocompatibility and anti-inflammatory effect of scaffold 11 Wound healing and angiogenic effect of the scaffold 14 Volume retention effect of the scaffold for fat grafting survival 15 In vivo evaluation of the scaffold for fat grafting survival 17 4. Conclusions 19 References 21 Abstract 26 Acknowledgements 28 | - |
dc.format.extent | viii, 28 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | "Fatgraft" | - |
dc.subject | "Allograftadiposematrix" | - |
dc.subject | "PLGA/MHmicrosphere" | - |
dc.subject | "Magnesiumhydroxide[Mg(OH)2]" | - |
dc.subject | "Angiogenesis" | - |
dc.subject | "fatsurvival" | - |
dc.subject.ddc | 610 | - |
dc.title | Fat Graft with Allograft Adipose Matrix and Magnesium Hydroxide-Incorporated PLGA Microspheres for Effective Soft Tissue Reconstruction | - |
dc.title.alternative | 효과적 연부조직 재건을 위한 동종지방기질 및 수산화마그네슘 함유 폴리(락틱-코-글리콜산) 미립구를 이용한 지방이식술 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.contributor.AlternativeAuthor | Dae Hee Kim | - |
dc.contributor.department | 의과대학 의학과 | - |
dc.description.degree | 박사 | - |
dc.date.awarded | 2022-08 | - |
dc.contributor.major | 의공학 | - |
dc.identifier.uci | I804:11032-000000172516 | - |
dc.identifier.holdings | 000000000048▲000000000055▲000000172516▲ | - |
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