Publications
Detailed Information
The Safety and Effectiveness of Ascorbyl Propyl Hyaluronate in Atopic Dermatitis : 아토피 피부염 환자에 적용한 Ascorbyl Propyl Hyaluronate 의 안전성 및 유효성에 관한 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 백롱민 | - |
dc.contributor.author | 김지훈 | - |
dc.date.accessioned | 2022-12-29T08:55:52Z | - |
dc.date.available | 2022-12-29T08:55:52Z | - |
dc.date.issued | 2022 | - |
dc.identifier.other | 000000172150 | - |
dc.identifier.uri | https://hdl.handle.net/10371/188384 | - |
dc.identifier.uri | https://dcollection.snu.ac.kr/common/orgView/000000172150 | ko_KR |
dc.description | 학위논문(박사) -- 서울대학교대학원 : 의과대학 의학과, 2022. 8. 백롱민. | - |
dc.description.abstract | Atopic dermatitis (AD) is chronic relapsing inflammation of the skin
associated with severe itching. It is results in structural and functional damage of skin. Recently, ascorbic acid (AA) wa s reported to plays an important role i n AD. The AD symptom was alleviated, when the decreased AA was supplemented in the sk in. Ascorbyl propyl hyaluronate (APH), a hyaluronate ascorbic acid derivative, is a cosmetic ingredient improved stability and skin permeability. In this study, we aimed to assess the safety and efficacy of APH in AD patients. W e demonstrated that APH is improved thermal stability compar ed with ascorbic acid. APH treatment did not affect cell morphology and viability of immortalized human keratinocytes HaCaT and human dermal fibroblasts (HDF). Moreover, APH had free radical scavenging effect and reduced inflammatory cytokine such as TNF α and IL 1 α . Risk assessment of APH were evaluated by skin irritation and sensitization test. There was no adverse effect. We observed that topical application of APH improved EASI eczema area and severity index )), skin hydration, trans epi dermal water loss and pruritu s of AD patients. These findings suggest that APH can be used as an active ingredient to a lleviate AD symptoms. | - |
dc.description.abstract | 아토피 피부염(AD)은 심한 가려움증을 동반하는 만성 재발성 염증성
피부질환이다. 이는 피부의 구조적 및 기능적 손상을 초래한다. 최근 아 스코르브산(AA)이 아토피 피부염에서 중요한 역할을 하는 것으로 알려 졌다. 피부에서 감소된 AA를 보충하면 아토피 피부염 증상이 완화되었 다. 히알루론산-아스코르빈산 유도체인 아스코르빌 프로필 히알루론산 (APH)은 안정성과 피부 투과성을 개선한 화장품 성분이다. 본 연구에 서는 아토피 피부염 환자에게서 APH의 안전성과 유효성을 평가하고자 하였다. 저자는 APH가 아스코르브산에 비해 열안정성이 향상된 것을 입증했다. APH 처리는 HaCaT 및 인간 진피 섬유아세포(HDF)의 세포 형태와 생존력에 영향을 미치지 않았다. 또한, APH는 자유-라디칼 제거 효과가 있었고 TNF-α 및 IL-1α와 같은 염증성 사이토카인을 감소 시켰다. APH의 위해성 평가는 피부 자극 및 과민성 시험으로 평가하였 으며, 부작용은 관찰되지 않았다. 저자는 APH의 국소 적용이 아토피 피 부염 환자의 습진 면적 및 중증도 지수, 피부 보습, 경피 수분 손실 및 가려움증의 개선을 가져온다는 것을 관찰했다. 이러한 연구 결과는 APH가 아토피 피부염 증상을 완화시키는 활성 성분으로 사용될 수 있 음을 시사한다. | - |
dc.description.tableofcontents | Introduction 1
Material and Methods 5 Results 20 Discussion 28 References 70 Abstract in Korean 74 LIST OF FIGURES 35 Figure 1. Chemical structure and thermal stability of APH 35 Figure 2. Free radical scavenging activity of APH 36 Figure 3. Permeability of APH through artificial membranes 37 Figure 4. The effect of APH on the cell viability and morphology 38-40 Figure 5. Anti inflammatory effect of APH 41-45 Figure 6. Assessment of the skin sensitization study schedule 46 Figure 7. Eczema Area and Severity Index (EASI) score assessment 47 Figure 8. Skin hydration assessment 48 Figure 9. Trans epidermal water loss (TEWL) assessment 49 Figure 10. Pruritus assessment 50 Figure 11. Clinical photography 51 LIST OF TABLES 52 Table 1. Measurement of flux and permeability coefficient (Kp) 52 Table 2. Skin characteristics of skin irritation subject(n=31) 53 Table 3. Change of Eczema area and severity index (EASI) Score 54 Table 4. Change of skin hydration 55 Table 5. Change of Trans-epidermal water loss (TEWL) 56 Table 6. Change of pruritus 57 Table 7. Q. 1) How satisfied are you with your product overall ? 58 Table 8. Q. 2 1) Are you satisfied with the overall a topic dermatitis improvement after using the test product ? 59 Table 9. Q. 2 2) Have you notice d an improvement in your skin's dryness after using the test product ? 60 Table 10. Q. 2 3) Are you satisfied with improvement in pruritus after using the test product ? 61 Table 11. Q. 2 4) Did your skin become smooth after using the test product ? 62 Table 12. Q. 2 5) Is the test product more effective than the one you used recently ? 63 Table 13. Q. 2 6) Will you recommend this product to people around you ? 64 Table 14. Q. 3) Have you experienced any discomfort or adverse reactions while using the test product 65 Supplementary Table 1 66 Supplementary Table 2 67 Supplementary Table 3 68 Supplementary Table 4 69 | - |
dc.format.extent | vi, 74 | - |
dc.language.iso | eng | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Atopicdermatitis | - |
dc.subject | Ascorbicacid | - |
dc.subject | Hyaluronicacid | - |
dc.subject | Antioxidants | - |
dc.subject | Antiinflammation | - |
dc.subject.ddc | 610 | - |
dc.title | The Safety and Effectiveness of Ascorbyl Propyl Hyaluronate in Atopic Dermatitis | - |
dc.title.alternative | 아토피 피부염 환자에 적용한 Ascorbyl Propyl Hyaluronate 의 안전성 및 유효성에 관한 연구 | - |
dc.type | Thesis | - |
dc.type | Dissertation | - |
dc.contributor.AlternativeAuthor | KIM, JIHOON | - |
dc.contributor.department | 의과대학 의학과 | - |
dc.description.degree | 박사 | - |
dc.date.awarded | 2022-08 | - |
dc.contributor.major | 성형외과학 | - |
dc.identifier.uci | I804:11032-000000172150 | - |
dc.identifier.holdings | 000000000048▲000000000055▲000000172150▲ | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.