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Potential Role of Heme Oxygenase-1 in the Resolution of Experimentally Induced Colitis through Regulation of Macrophage Polarization
DC Field | Value | Language |
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dc.contributor.author | Gwak, Shin-Young | - |
dc.contributor.author | Kim, Su-Jung | - |
dc.contributor.author | Park, Jeongmin | - |
dc.contributor.author | Kim, Seung Hyeon | - |
dc.contributor.author | Joe, Yeonsoo | - |
dc.contributor.author | Lee, Ha-Na | - |
dc.contributor.author | Kim, Wonki | - |
dc.contributor.author | Muna, Ishrat Aklima | - |
dc.contributor.author | Na, Hye-Kyung | - |
dc.contributor.author | Chung, Hun Taeg | - |
dc.contributor.author | Surh, Young-Joon | - |
dc.date.accessioned | 2023-03-20T08:44:48Z | - |
dc.date.available | 2023-03-20T08:44:48Z | - |
dc.date.created | 2022-04-18 | - |
dc.date.created | 2022-04-18 | - |
dc.date.created | 2022-04-18 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.citation | Gut and Liver, Vol.16 No.2, pp.246-258 | - |
dc.identifier.issn | 1976-2283 | - |
dc.identifier.uri | https://hdl.handle.net/10371/189494 | - |
dc.description.abstract | Background/Aims: Heme oxygenase-1 (HO-1) plays a central role in cellular defense against inflammatory insults, and its induction in macrophages potentiates their efferocytic activity. In this study, we explored the potential role of macrophage HO-1 in the resolution of experimentally induced colitis. Methods: To induce colitis, male C57BL/6 mice were treated with 2% dextran sulfate sodium (DSS) in the drinking water for 7 days. To investigate efferocytosis, apoptotic colon epithelial CCD 841 CoN cells were coincubated with bone marrow-derived macrophages (BMDMs). Results: Administration of the HO-1 inhibitor zinc protoporphyrin IX (ZnPP) blunted the resolution of DSS-induced intestinal inflammation and expression of the proresolving M2 macrophage marker CD206. BMDMs treated with apoptotic colonic epithelial cells showed significantly elevated expression of HO-1 and its regulator Nrf2. Under the same experimental conditions, the proportion of CD206-expressing macrophages was also enhanced. ZnPP treatment abrogated the upregulation of CD206 expression in BMDMs engulfing apoptotic colonic epithelial cells. This result was verified with BMDMs isolated from HO-1-knockout mice. BMDMs, when stimulated with lipopolysaccharide, exhibited increased expression of CD86, a marker of M1 macrophages. Coculture of lipopolysaccharide-stimulated BMDMs with apoptotic colonic epithelial cell debris dampened the expression of CD86 as well as the pro-inflammatory cytokines in an HO-1-dependent manner. Genetic ablation as well as pharmacologic inhibition of HO-1 significantly reduced the proportion of efferocytic BMDMs expressing the scavenger receptor CD36. Conclusions: HO-1 plays a key role in the resolution of experimentally induced colitis by modulating the polarization of macrophages. | - |
dc.language | 영어 | - |
dc.publisher | 거트앤리버 발행위원회 | - |
dc.title | Potential Role of Heme Oxygenase-1 in the Resolution of Experimentally Induced Colitis through Regulation of Macrophage Polarization | - |
dc.type | Article | - |
dc.identifier.doi | 10.5009/gnl210058 | - |
dc.citation.journaltitle | Gut and Liver | - |
dc.identifier.wosid | 000717782700001 | - |
dc.identifier.scopusid | 2-s2.0-85126830285 | - |
dc.citation.endpage | 258 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 246 | - |
dc.citation.volume | 16 | - |
dc.identifier.kciid | ART002820900 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Surh, Young-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CARBON-MONOXIDE | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | INTESTINAL HOMEOSTASIS | - |
dc.subject.keywordPlus | ULCERATIVE-COLITIS | - |
dc.subject.keywordPlus | APOPTOTIC CELLS | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | EFFEROCYTOSIS | - |
dc.subject.keywordPlus | CLEARANCE | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordAuthor | Acute colitis | - |
dc.subject.keywordAuthor | Dextran sulfate sodium | - |
dc.subject.keywordAuthor | Efferocytosis | - |
dc.subject.keywordAuthor | Macrophage polarization | - |
dc.subject.keywordAuthor | Resolution of inflammation | - |
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