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Immunomodulatory effects of a bioactive compound isolated from dryopteris crassirhizoma on the grass carp ctenopharyngodon idella

Cited 20 time in Web of Science Cited 18 time in Scopus
Authors

Chi, Cheng; Giri, Sib Sankar; Jun, Jin Woo; Kim, Hyoun Joong; Yun, Saekil; Kim, Sang Guen; Park, Se Chang

Issue Date
2016-01
Publisher
Hindawi Publishing Corporation
Citation
Journal of Immunology Research, Vol.2016, p. 3068913
Abstract
In the present study, we investigated effects of compound kaempferol 3-a-L-(4-O-acetyl) rhamnopyranoside-7-a-L-rhamnopyranoside (SA) isolated from Dryopteris crassirhizoma during immune-related gene expression in Ctenopharyngodon idella head kidney macrophages (CIHKM). The expression of immune-related genes (IL-1 beta, TNF-alpha, MyD88, and Mx1) were investigated using realtime PCR at 2 h, 8 h, 12 h, and 24 h after incubation with 1, 10, and 50 mu g mL(-1) of SA. Furthermore, fish were injected intraperitoneally with 100 mu L of SA, and immune parameters such as lysozyme activity, complement C3, SOD, phagocytic activity, and IgM level were examined at 1, 2, and 3 weeks after injection. The differential expression of cytokines was observed after exposure to SA. IL-1 beta genes displayed significant expression at 2 and 8 h after exposure to 1-10 mu g mL(-1) of SA. SA also induced gene expression of cytokines such as MyD88, Mx1, and TNF-alpha. Furthermore, enhanced immune parameters in grass carp confirmed the immunomodulatory activity of SA. Interestingly, this compound has no toxic effect on CIHKM cells as tested by MTT assay. In addition, fish immunised with 10 mu g mL(-1) of SA exhibited maximum resistance against Aeromonas hydrophila infection. These results suggest that SA has the potential to stimulate immune responses in grass carp.
ISSN
2314-8861
URI
https://hdl.handle.net/10371/190165
DOI
https://doi.org/10.1155/2016/3068913
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  • College of Veterinary Medicine
  • Department of Veterinary Medicine
Research Area Bacteriophage Therapy, Veterinary Medicine, Veterinary Microbiology

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