Publications
Detailed Information
Phosphoglycerate kinase 1 protects against ischemic damage in the gerbil hippocampus
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hahn, Kyu Ri | - |
dc.contributor.author | Kwon, Hyun Jung | - |
dc.contributor.author | Yoon, Yeo Sung | - |
dc.contributor.author | Kim, Dae Won | - |
dc.contributor.author | Hwang, In Koo | - |
dc.date.accessioned | 2023-04-19T00:03:30Z | - |
dc.date.available | 2023-04-19T00:03:30Z | - |
dc.date.created | 2023-01-20 | - |
dc.date.issued | 2022-11 | - |
dc.identifier.citation | Aging-us, Vol.14 No.22, pp.8886-8899 | - |
dc.identifier.issn | 1945-4589 | - |
dc.identifier.uri | https://hdl.handle.net/10371/190173 | - |
dc.description.abstract | Phosphoglycerate kinase 1 (PGK1) is a metabolic enzyme that converts 1,3-diphosphoglycerate to 3phosphoglycerate. In the current study, we synthesized a PEP-1-PGK1 fusion protein that can cross the bloodbrain barrier and cell membrane, and the effects of PEP-1-PGK1 against oxidative stress were investigated HT22 cells and ischemic gerbil brain. The PEP-1-PGK1 protein and its control protein (Con-PGK1) were treated and permeability was evaluated HT22 cells. The PEP-1-PGK1 was introduced into HT22 cells depending on its concentration and incubation time and was gradually degraded over 36 h after treatment. PEP-1-PGK1, but not Con-PGK1, significantly ameliorated H2O2-induced cell damage and reactive oxygen species formation in HT22 cells. Additionally, PEP-1-PGK1, but not Con-PGK1, mitigated ischemia-induced hyperlocomotion 1 d after ischemia and 4 d after ischemia of neuronic cell death. PEP-1-PGK1 treatment significantly alleviated the raised lactate and succinate dehydrogenase activities in the early (15 min to 6 h) and late (4 and 7 d) stages of ischemia, respectively. In addition, PEP-1-PGK1 treatment ameliorated the decrease in ATP and pH levels in the late stage (2-7 d) of ischemia. Nuclear factor erythroid-2-related factor 2 (Nrf2) levels accelerated the ischemiainduced increase in the hippocampus 1 d after ischemia after PEP-1-PGK1 treatment. Neuroprotective and ameliorative effects were prominent at a low concentration (0.1 mg/kg), but not at a high concentration (1 mg/kg), of PEP-1-PGK1. Collectively, low concentrations of PEP-1-PGK1 prevented neuronal stress by increasing energy production. | - |
dc.language | 영어 | - |
dc.publisher | IMPACT JOURNALS LLC | - |
dc.title | Phosphoglycerate kinase 1 protects against ischemic damage in the gerbil hippocampus | - |
dc.type | Article | - |
dc.identifier.doi | 10.18632/aging.204343 | - |
dc.citation.journaltitle | Aging-us | - |
dc.identifier.wosid | 000892356700003 | - |
dc.identifier.scopusid | 2-s2.0-85143551881 | - |
dc.citation.endpage | 8899 | - |
dc.citation.number | 22 | - |
dc.citation.startpage | 8886 | - |
dc.citation.volume | 14 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Yoon, Yeo Sung | - |
dc.contributor.affiliatedAuthor | Hwang, In Koo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FOREBRAIN ISCHEMIA | - |
dc.subject.keywordPlus | AEROBIC GLYCOLYSIS | - |
dc.subject.keywordPlus | CEREBRAL-ISCHEMIA | - |
dc.subject.keywordPlus | NEURONAL SURVIVAL | - |
dc.subject.keywordPlus | PGK1 | - |
dc.subject.keywordPlus | NRF2 | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | phosphoglycerate kinase 1 | - |
dc.subject.keywordAuthor | lactate | - |
dc.subject.keywordAuthor | ATP | - |
dc.subject.keywordAuthor | Nrf2 | - |
dc.subject.keywordAuthor | neuroprotection | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.