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Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo, Yeon-Jee | - |
dc.contributor.author | Kang, Min-Ji | - |
dc.contributor.author | Perinpanayagam, Hiran | - |
dc.contributor.author | Park, Joo-Cheol | - |
dc.contributor.author | Baek, Seung-Ho | - |
dc.contributor.author | Kum, Kee-Yeon | - |
dc.date.accessioned | 2023-04-19T00:58:48Z | - |
dc.date.available | 2023-04-19T00:58:48Z | - |
dc.date.created | 2021-08-23 | - |
dc.date.created | 2021-08-23 | - |
dc.date.created | 2021-08-23 | - |
dc.date.created | 2021-08-23 | - |
dc.date.created | 2021-08-23 | - |
dc.date.issued | 2021-08 | - |
dc.identifier.citation | Applied Sciences-basel, Vol.11 No.15, p. 6836 | - |
dc.identifier.issn | 2076-3417 | - |
dc.identifier.uri | https://hdl.handle.net/10371/190321 | - |
dc.description.abstract | This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (n = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (n = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (n = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (p < 0.05), without interactions with EDTA (p > 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration. | - |
dc.language | 영어 | - |
dc.publisher | MDPI AG | - |
dc.title | Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/app11156836 | - |
dc.citation.journaltitle | Applied Sciences-basel | - |
dc.identifier.wosid | 000681953100001 | - |
dc.identifier.scopusid | 2-s2.0-85111928726 | - |
dc.citation.number | 15 | - |
dc.citation.startpage | 6836 | - |
dc.citation.volume | 11 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Park, Joo-Cheol | - |
dc.contributor.affiliatedAuthor | Kum, Kee-Yeon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordAuthor | dentin | - |
dc.subject.keywordAuthor | human dental pulp stem cells | - |
dc.subject.keywordAuthor | live-cell imaging | - |
dc.subject.keywordAuthor | MTT assay | - |
dc.subject.keywordAuthor | non-thermal atmospheric pressure plasma | - |
dc.subject.keywordAuthor | scanning electron microscopy | - |
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