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Efficacy of PLGA microparticle-encapsulated formalin-killed Aeromonas hydrophila cells as a single-shot vaccine against A. hydrophila infection

Cited 22 time in Web of Science Cited 27 time in Scopus

Yun, Saekil; Jun, Jin Woo; Giri, Sib Sankar; Kim, Hyoun Joong; Chi, Cheng; Kim, Sang Geun; Kim, Sang Wha; Park, Se Chang

Issue Date
Elsevier BV
Vaccine, Vol.35 No.32, pp.3959-3965
Control and prevention of disease is a high priority in aquaculture, and vaccination is important to prevent outbreaks. Here, poly((D),(L)-lactide-co-glycolic acid) (PLGA) microparticles (MPs) approximately in diameter were used to encapsulate and deliver Aeromonas hydrophila formalin-killed cells (FKC) as an antigen, and the innate and adaptive immune responses of cyprinid loaches and common carp were assessed following vaccination. The antigen was confirmed to be well encapsulated by scanning electron microscopy analysis of PLGA MP sections. Blood and head kidney specimens were collected and analyzed for bacterial agglutination activity and relative mRNA expression of immune-related genes (IL-1 beta, IL-10, TNF-alpha, lysozyme C, TGF-beta, and IgM) at 2, 4, 6, and 8 weeks post vaccination (wpv). For both fish species, the curve of antibody titer over time was shallower in the PLGA group than the FKC group. These titers in loaches and carp were very similar in the two vaccination groups until 8 and 6 wpv, respectively, but differences were subsequently noted in both species until the end of experiment. Loathes and carp were then challenged with A. hydrophila at 12 and 20 wpv, and 10 and 14 wpv, respectively, and relative survival rates were calculated. For both species, the PLGA groups demonstrated higher survival rates at all time points. Relative expression of IL-1 beta and TNF-alpha mRNA was significantly upregulated in the PLGA group at 2 and 4 wpv. Moreover, PLGA-MP vaccination increased relative mRNA levels of lysozyme C and IgM, which were significantly higher than those observed with FKC treatment at 2 wpv and 4, 6, and 8 wpv, respectively. In conclusion, PLGA-MP vaccines have the potential to induce longer and more potent immune responses than FKCs alone, and protect both cyprinid loaches and common carp with greater efficiency. (C) 2017 Elsevier Ltd. All rights reserved.
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  • College of Veterinary Medicine
  • Department of Veterinary Medicine
Research Area Bacteriophage Therapy, Veterinary Medicine, Veterinary Microbiology


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