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Characterization and complete genome sequence of the Shigella bacteriophage pSf-1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jun, Jin Woo | - |
dc.contributor.author | Kim, Ji Hyung | - |
dc.contributor.author | Shin, Sang Phil | - |
dc.contributor.author | Han, Jee Bun | - |
dc.contributor.author | Chai, Ji Young | - |
dc.contributor.author | Park, Se Chang | - |
dc.date.accessioned | 2023-04-19T06:59:26Z | - |
dc.date.available | 2023-04-19T06:59:26Z | - |
dc.date.created | 2021-04-15 | - |
dc.date.created | 2021-04-15 | - |
dc.date.created | 2021-04-15 | - |
dc.date.created | 2021-04-15 | - |
dc.date.issued | 2013-12 | - |
dc.identifier.citation | Research in Microbiology, Vol.164 No.10, pp.979-986 | - |
dc.identifier.issn | 0923-2508 | - |
dc.identifier.uri | https://hdl.handle.net/10371/190706 | - |
dc.description.abstract | Shigellosis is a global health problem, and Shigella flexneri is the major cause of this disease. In this study, we isolated a virulent Siphoviridae bacteriophage (phage), pSf-1, that infects S. flexneri. This phage was isolated from the Han River in Korea and was found to infect S. flexneri, Shigella boydii, and Shigella sonnei. One-step growth analysis revealed that this phage has a short latent period (10 mm) and a large burst size (86.86 PFU/cell), indicating that pSf-1 has good host infectivity and effective lytic activity. The double-stranded DNA genome of pSf-1 is composed of 51,821 bp with a G C content of 44.02%. The genome encodes 94 putative ORFs, 71 putative promoters, and 60 transcriptional terminator regions. Genome sequence analysis of pSf-1 and comparative analysis with the homologous Shigella phage Shfl1 revealed that there is a high degree of similarity between pSf-1 and Shfl1 in 54 of the 94 ORFs of pSf-1. The results of this investigation indicate that pSf-1 is a novel Shigella phage and that this phage might have potential uses against shigellosis. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Characterization and complete genome sequence of the Shigella bacteriophage pSf-1 | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.resmic.2013.08.007 | - |
dc.citation.journaltitle | Research in Microbiology | - |
dc.identifier.wosid | 000329379400002 | - |
dc.identifier.scopusid | 2-s2.0-84889092016 | - |
dc.citation.endpage | 986 | - |
dc.citation.number | 10 | - |
dc.citation.startpage | 979 | - |
dc.citation.volume | 164 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Se Chang | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SONNEI-GASTROENTERITIS | - |
dc.subject.keywordPlus | KLEBSIELLA-PNEUMONIAE | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | PHAGE THERAPY | - |
dc.subject.keywordPlus | FOOD SAFETY | - |
dc.subject.keywordPlus | HOST-RANGE | - |
dc.subject.keywordPlus | OUTBREAK | - |
dc.subject.keywordPlus | FLEXNERI | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | CONSUMPTION | - |
dc.subject.keywordAuthor | Shigellosis | - |
dc.subject.keywordAuthor | Shigella flexneri | - |
dc.subject.keywordAuthor | Siphoviridae | - |
dc.subject.keywordAuthor | Bacteriophage | - |
dc.subject.keywordAuthor | pSf-1 | - |
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