Publications

Detailed Information

Sec62 Protein Mediates Membrane Insertion and Orientation of Moderately Hydrophobic Signal Anchor Proteins in the Endoplasmic Reticulum (ER)

DC Field Value Language
dc.contributor.authorReithinger, Johannes H.-
dc.contributor.authorKim, Ji Eun Hani-
dc.contributor.authorKim, Hyun-
dc.date.accessioned2023-04-19T07:00:10Z-
dc.date.available2023-04-19T07:00:10Z-
dc.date.created2021-04-26-
dc.date.created2021-04-26-
dc.date.issued2013-06-
dc.identifier.citationJournal of Biological Chemistry, Vol.288 No.25, pp.18058-18067-
dc.identifier.issn0021-9258-
dc.identifier.urihttps://hdl.handle.net/10371/190722-
dc.description.abstractNascent chains are known to be targeted to the endoplasmic reticulum membrane either by a signal recognition particle (SRP)-dependent co-translational or by an SRP-independent post-translational translocation route depending on signal sequences. Using a set of model and cellular proteins carrying an N-terminal signal anchor sequence of controlled hydrophobicity and yeast mutant strains defective in SRP or Sec62 function, the hydrophobicity-dependent targeting efficiency and targeting pathway preference were systematically evaluated. Our results suggest that an SRP-dependent co-translational and an SRP-independent post-translational translocation are not mutually exclusive for signal anchor proteins and that moderately hydrophobic ones require both SRP and Sec62 for proper targeting and translocation to the endoplasmic reticulum. Further, defect in Sec62 selectively reduced signal sequences inserted in an N-in-C-out (type II) membrane topology, implying an undiscovered role of Sec62 in regulating the orientation of the signal sequence in an early stage of translocation.-
dc.language영어-
dc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.-
dc.titleSec62 Protein Mediates Membrane Insertion and Orientation of Moderately Hydrophobic Signal Anchor Proteins in the Endoplasmic Reticulum (ER)-
dc.typeArticle-
dc.identifier.doi10.1074/jbc.M113.473009-
dc.citation.journaltitleJournal of Biological Chemistry-
dc.identifier.wosid000320721900012-
dc.identifier.scopusid2-s2.0-84880071444-
dc.citation.endpage18067-
dc.citation.number25-
dc.citation.startpage18058-
dc.citation.volume288-
dc.description.isOpenAccessY-
dc.contributor.affiliatedAuthorKim, Hyun-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusPREPRO-ALPHA-FACTOR-
dc.subject.keywordPlusSEQUENCE ORIENTATION-
dc.subject.keywordPlusTARGETING PATHWAY-
dc.subject.keywordPlusMOLECULAR CODE-
dc.subject.keywordPlusYEAST-
dc.subject.keywordPlusTRANSPORT-
dc.subject.keywordPlusCOMPLEX-
dc.subject.keywordPlusTRANSLOCATION-
dc.subject.keywordPlusRECOGNITION-
dc.subject.keywordPlusDOMAIN-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share