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Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts

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dc.contributor.authorChoresca, Casiano H., Jr.-
dc.contributor.authorKoo, Ok Jae-
dc.contributor.authorOh, Hyun Ju-
dc.contributor.authorHong, So Gun-
dc.contributor.authorGomez, Dennis K.-
dc.contributor.authorKim, Ji Hyung-
dc.contributor.authorLee, Byeong Chun-
dc.contributor.authorPark, Se Chang-
dc.date.accessioned2023-04-19T07:05:50Z-
dc.date.available2023-04-19T07:05:50Z-
dc.date.created2021-04-15-
dc.date.created2021-04-15-
dc.date.created2021-04-15-
dc.date.created2021-04-15-
dc.date.created2021-04-15-
dc.date.created2021-04-15-
dc.date.issued2009-01-
dc.identifier.citationCell Biology International, Vol.33 No.1, pp.65-70-
dc.identifier.issn1065-6995-
dc.identifier.urihttps://hdl.handle.net/10371/190780-
dc.description.abstractOne of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P < 0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15 mu M) induce cell cycle arrest at the G0/G1 phase. Crown Copyright (C) 2008 Published by Elsevier Ltd on behalf of International Federation for Cell Biology. All rights reserved.-
dc.language영어-
dc.publisherAcademic Press-
dc.titleDifferent culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts-
dc.typeArticle-
dc.identifier.doi10.1016/j.cellbi.2008.09.015-
dc.citation.journaltitleCell Biology International-
dc.identifier.wosid000261949000008-
dc.identifier.scopusid2-s2.0-57649195418-
dc.citation.endpage70-
dc.citation.number1-
dc.citation.startpage65-
dc.citation.volume33-
dc.description.isOpenAccessN-
dc.contributor.affiliatedAuthorLee, Byeong Chun-
dc.contributor.affiliatedAuthorPark, Se Chang-
dc.type.docTypeArticle-
dc.description.journalClass1-
dc.subject.keywordPlusMEDAKA ORYZIAS-LATIPES-
dc.subject.keywordPlusADULT SOMATIC-CELLS-
dc.subject.keywordPlusNUCLEAR TRANSFER-
dc.subject.keywordPlusSKIN FIBROBLASTS-
dc.subject.keywordPlusSERUM STARVATION-
dc.subject.keywordPlusENUCLEATED EGGS-
dc.subject.keywordPlusKINASE-ACTIVITY-
dc.subject.keywordPlusROSCOVITINE-
dc.subject.keywordPlusCALVES-
dc.subject.keywordPlusFISH-
dc.subject.keywordAuthorCell cycle synchronization-
dc.subject.keywordAuthorG0/G1 phase-
dc.subject.keywordAuthorGoldfish-
dc.subject.keywordAuthorSerum starvation-
dc.subject.keywordAuthorCulture to confluence-
dc.subject.keywordAuthorRoscovitine-
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  • College of Veterinary Medicine
  • Department of Veterinary Medicine
Research Area Bacteriophage Therapy, Veterinary Medicine, Veterinary Microbiology

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