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Imaging single mRNA dynamics in live neurons and brains
DC Field | Value | Language |
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dc.contributor.author | Moon, H. C. | - |
dc.contributor.author | Park, H. Y. | - |
dc.date.accessioned | 2023-04-19T08:59:56Z | - |
dc.date.available | 2023-04-19T08:59:56Z | - |
dc.date.created | 2018-08-17 | - |
dc.date.created | 2018-08-17 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | Methods in Enzymology, Vol.572, pp.51-64 | - |
dc.identifier.issn | 0076-6879 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191251 | - |
dc.description.abstract | RNA is a key player in the process of gene expression. Whereas fluorescence in situ hybridization allows single mRNA imaging in fixed cells, the MS2-GFP labeling technique enables the observation of mRNA dynamics in living cells. Recently, two genetically engineered mouse models have been developed for the application of the MS2-GFP system in live animals. First, the Actb-MBS mouse was generated by knocking in 24 repeats of the MS2 stem-loop sequence in the 3' untranslated region of the beta-actin gene. Second, the MCP mouse was made to express the NLS-HA-MCP-GFP transgene in all cell types. By crossing Actb-MBS and MCP mice, a double homozygous mouse line, MCP x MBS, was established to visualize endogenous beta-actin mRNA labeled with multiple green fluorescent proteins. By imaging hippocampal neurons or brain slices from MCP x MBS mice, the dynamics of mRNA, such as transcription, transport, and localization, can be studied at single mRNA resolution. In this chapter, we explain the basics of MCP x MBS mice and describe methods for utilizing these animals. | - |
dc.language | 영어 | - |
dc.publisher | Academic Press | - |
dc.title | Imaging single mRNA dynamics in live neurons and brains | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/bs.mie.2016.02.015 | - |
dc.citation.journaltitle | Methods in Enzymology | - |
dc.identifier.wosid | 000382016100003 | - |
dc.identifier.scopusid | 2-s2.0-84962199682 | - |
dc.citation.endpage | 64 | - |
dc.citation.startpage | 51 | - |
dc.citation.volume | 572 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, H. Y. | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | LOCAL PROTEIN-SYNTHESIS | - |
dc.subject.keywordPlus | QUANTITATIVE RT-PCR | - |
dc.subject.keywordPlus | SYNAPTIC PLASTICITY | - |
dc.subject.keywordPlus | LATE MAINTENANCE | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | VISUALIZATION | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordAuthor | mRNA | - |
dc.subject.keywordAuthor | NeuronSingle-molecule imaging | - |
dc.subject.keywordAuthor | Brain slice | - |
dc.subject.keywordAuthor | Two-photon microscopy | - |
dc.subject.keywordAuthor | β-Actin | - |
dc.subject.keywordAuthor | MS2-GFP | - |
dc.subject.keywordAuthor | Transcription | - |
dc.subject.keywordAuthor | mRNA localization | - |
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