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Apocynin suppressed the nuclear factor-B pathway and attenuated lung injury in a rat hemorrhagic shock model
DC Field | Value | Language |
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dc.contributor.author | Choi, Seok Ho | - |
dc.contributor.author | Suh, Gil Joon | - |
dc.contributor.author | Kwon, Woon Yong | - |
dc.contributor.author | Kim, Kyung Su | - |
dc.contributor.author | Park, Min Ji | - |
dc.contributor.author | Kim, Taegyun | - |
dc.contributor.author | Ko, Jeong In | - |
dc.date.accessioned | 2023-04-20T04:27:45Z | - |
dc.date.available | 2023-04-20T04:27:45Z | - |
dc.date.created | 2018-08-20 | - |
dc.date.issued | 2017-03 | - |
dc.identifier.citation | Journal of Trauma and Acute Care Surgery, Vol.82 No.3, pp.566-574 | - |
dc.identifier.issn | 2163-0755 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191319 | - |
dc.description.abstract | BACKGROUND The aim of this study was to investigate whether a nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (Nox) inhibitor, apocynin, reduces reactive oxygen species (ROS) production, suppresses the nuclear factor B (NF-B) pathway, attenuates lung injury, and improves survival in rat hemorrhagic shock (HS) model. METHODS Blood was drawn from male Sprague-Dawley rats (290-340 g) to maintain a mean arterial pressure of 20-25 mm Hg for 40 minutes. The rats were resuscitated with the drawn blood, and a vehicle (HS), a low dose of apocynin (20 mg/kg, LD-Apo), or a high dose of apocynin (40 mg/kg, HD-Apo) was administered intraperitoneally. The survival of the rats was observed for 72 hours. Then, a separated set of rats was euthanized at 6 hours post-HS induction. We measured gp91-phox (Nox2) expression, Nox activity, cytoplasmic phosphorylated inhibitor B- (p-IB-) expression, NF-B p65 DNA-binding activity, tumor necrosis factor- (TNF-) and interleukin-6 (IL-6) gene expressions, malondialdehyde (MDA) level, myeloperoxidase (MPO) activity, and histological damage in the lung tissues. RESULTS The survival rates of the sham, HS, HS + LD-Apo, and HS + HD-Apo groups were 100% (5/5), 30% (3/10), 40% (4/10), and 70% (7/10), respectively. A high dose of apocynin decreased gp91-phox expression, Nox activity, and MDA level in the lung tissues during HS and resuscitation. It also decreased p-IB- expression, NF-B p65 DNA-binding activity, TNF- and IL-6 gene expressions, and MPO activity in the lung tissues and attenuated histological lung injuries. However, a low dose of apocynin failed to show these benefits. CONCLUSIONS The administration of a high dose of apocynin inhibited Nox2 expression and Nox activity, reduced lipid peroxidation, suppressed the NF-B pathway and subsequent pro-inflammatory cytokines transcription in the lung tissues, and attenuated lung injury during HS and resuscitation in rats. | - |
dc.language | 영어 | - |
dc.publisher | Lippincott Williams and Wilkins | - |
dc.title | Apocynin suppressed the nuclear factor-B pathway and attenuated lung injury in a rat hemorrhagic shock model | - |
dc.type | Article | - |
dc.identifier.doi | 10.1097/TA.0000000000001337 | - |
dc.citation.journaltitle | Journal of Trauma and Acute Care Surgery | - |
dc.identifier.wosid | 000395595300018 | - |
dc.identifier.scopusid | 2-s2.0-85007477155 | - |
dc.citation.endpage | 574 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 566 | - |
dc.citation.volume | 82 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Suh, Gil Joon | - |
dc.contributor.affiliatedAuthor | Kwon, Woon Yong | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | FACTOR-KAPPA-B | - |
dc.subject.keywordPlus | NADPH OXIDASE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | REPERFUSION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | NADPH oxidase | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | lung injury | - |
dc.subject.keywordAuthor | hemorrhagic shock | - |
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