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Efficient Cultivation Conditions for Human Limbal Epithelial Cells

Cited 9 time in Web of Science Cited 10 time in Scopus
Authors

Kim, Mee Kum; Lee, Jae Lim; Oh, Joo Youn; Shin, Mi Sun; Shin, Kyeong Seon; Wee, Won Ryang; Lee, Jin Hak; Park, Ki Sook; Son, Young Sook

Issue Date
2008-10
Publisher
대한의학회
Citation
Journal of Korean Medical Science, Vol.23 No.5, pp.864-869
Abstract
To compare the stem niche in different culture conditions of limbal epithelial cells, the suspended human limbal epithelial cells (HLECs) were seeded on the 3T3-pre-treated plates and the other suspended cells were plated on amniotic membranes (AMs) which were either cryo-preserved or freeze-dried. All were cultured for 10 to 12 days. Reverse transcription-polymerase chain reaction (RT-PCR) for ATP-binding casette, subfamily G, member 2 (ABCG2), p63, cytokeratin 12, and connexin 43 were performed in cultivated HLECs and their expression levels were compared. The mRNA expression of all markers examined showed no statistically significant differences between the cells on cryo-preserved and on freeze-dried AM. The expression of p63 and cytokeratin 12 in cultivated cells on AMs were significantly lower than those in 3T3-cocultured cells on RT-PCR and immunofluorescent staining. Cultivated HLECs on AMs showed reduced proliferation and differentiation while maintaining stem-property regardless of the preservative method of AM.
ISSN
1011-8934
URI
https://hdl.handle.net/10371/191686
DOI
https://doi.org/10.3346/jkms.2008.23.5.864
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  • College of Medicine
  • Department of Medicine
Research Area 각막 및 외안부 질환, 백내장

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