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Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, M. K. | - |
dc.contributor.author | Oh, J. Y. | - |
dc.contributor.author | Lee, H. I. | - |
dc.contributor.author | Ko, J. H. | - |
dc.contributor.author | Lee, H. J. | - |
dc.contributor.author | Lee, J. H. | - |
dc.contributor.author | Wee, W. R. | - |
dc.date.accessioned | 2023-04-26T08:09:28Z | - |
dc.date.available | 2023-04-26T08:09:28Z | - |
dc.date.created | 2023-04-26 | - |
dc.date.created | 2023-04-26 | - |
dc.date.created | 2023-04-26 | - |
dc.date.created | 2023-04-26 | - |
dc.date.issued | 2008-03 | - |
dc.identifier.citation | Transplantation Proceedings, Vol.40 No.2, pp.564-569 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://hdl.handle.net/10371/191691 | - |
dc.description.abstract | We admixed cultured porcine keratocytes or corneal endothelial cells in the presence of human sera or peripheral blood mononuclear cells (PBMCs) for 4 to 72 hours to investigate their immune-related susceptibilities to xeno-related rejection. We evaluated complement deposition at 48 hours by flow cytometry after staining with the C3 anti-goat cy3 antibody. The inhibition of proliferation of porcine corneal cells by human sera was examined using the 3-[4,5-dimethy/thiazol-2,5-dephenyl tetrazolium bromide (MTT) assay over 24 to 72 hours. The amount of (51)chromium (Cr)-release was estimated after a reaction between the porcine cells and human PBMCs for 4 hours. There was greater C3 deposition in keratocytes (60.2%) than in endothelial cells (26.9%; P = .05, Mann-Whitney U test). Both keratocytes and endothelial cells showed significant levels of proliferative inhibition over a period of 72 hours. The number of Cr-51-release cells on interleukin-2 addition was significantly higher among keratocytes (88.0%) than endothelial cells (51.4%) at a 1:100 target:effector ratio (P = .04, Mann-Whitney U test). Our present data suggested that porcine keratocytes might be key target cells in xeno-related rejections when the porcine cornea is transplanted to primates. | - |
dc.language | 영어 | - |
dc.publisher | Appleton & Lange | - |
dc.title | Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.transproceed.2007.12.024 | - |
dc.citation.journaltitle | Transplantation Proceedings | - |
dc.identifier.wosid | 000254695600071 | - |
dc.identifier.scopusid | 2-s2.0-40949092747 | - |
dc.citation.endpage | 569 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 564 | - |
dc.citation.volume | 40 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Oh, J. Y. | - |
dc.contributor.affiliatedAuthor | Lee, J. H. | - |
dc.contributor.affiliatedAuthor | Wee, W. R. | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CD4(+) T-CELLS | - |
dc.subject.keywordPlus | CORNEAL XENOGRAFTS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | ANTIBODIES | - |
dc.subject.keywordPlus | ANTIGENS | - |
dc.subject.keywordPlus | KIDNEYS | - |
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