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Screening of anaplastic lymphoma kinase rearrangement by immunohistochemistry in non-small cell lung cancer: Correlation with fluorescence in situ hybridization
DC Field | Value | Language |
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dc.contributor.author | Paik, Jin Ho | - |
dc.contributor.author | Choe, Gheeyoung | - |
dc.contributor.author | Kim, Hyojin | - |
dc.contributor.author | Choe, Ji-Young | - |
dc.contributor.author | Lee, Hyun Ju | - |
dc.contributor.author | Lee, Choon-Taek | - |
dc.contributor.author | Lee, Jong Seok | - |
dc.contributor.author | Jheon, Sanghoon | - |
dc.contributor.author | Chung, Jin-Haeng | - |
dc.date.accessioned | 2023-05-08T00:52:14Z | - |
dc.date.available | 2023-05-08T00:52:14Z | - |
dc.date.created | 2020-08-19 | - |
dc.date.created | 2020-08-19 | - |
dc.date.issued | 2011-03 | - |
dc.identifier.citation | Journal of Thoracic Oncology, Vol.6 No.3, pp.466-472 | - |
dc.identifier.issn | 1556-0864 | - |
dc.identifier.uri | https://hdl.handle.net/10371/192120 | - |
dc.description.abstract | Background: The use of a standard immunohistochemistry (IHC) assay to detect the anaplastic lymphoma kinase (ALK) protein in lung cancer is challenging. There are no universally accepted, evidence-based guidelines on identifying patients with ALK-rearranged lung cancer using IHC. Methods: We retrospectively reviewed 465 resected specimens of non-small cell lung cancer using a tissue microarray as a test set. ALK protein expression using IHC with 5A4 monoclonal antibody (Novocastra) and ALK gene rearrangement using fluorescence in situ hybridization (FISH) with dual-color break-apart probes (Abbott molecular) were examined. Immunoreactivity was scored as 0, 1, 2, or 3, and the results were compared with the FISH results. A diagnostic algorithm was derived from the correlation of the IHC and FISH results and applied to an additional 187 adenocarcinoma samples used as a validation set. Results: In the test set, ALK protein expression was detected in 40 patients (40/465, 8.6%), consisting of IHC scores of 1 (n = 14), 2 (n = 10), and 3 (n = 16), whereas 19 patients (19/453, 4.2%) were FISH-positive. All the FISH-positive patients were assigned IHC scores of 2 or 3. All the patients with ALK IHC scores of 3 were FISH-positive, those with scores of 0 or 1 were FISH-negative, and those with scores of 2 were FISH variable. In the validation set, ALK protein expression was detected in 14 patients (scores of 1, n = 2; scores of 2, n = 6; and scores of 3, n = 6), of which nine patients (9/187, 4.8%) were FISH-positive. All the patients with IHC scores of 0 or 1 were FISH-negative, and those with scores of 3 were FISH-positive. Among the patients with IHC scores of 2, three (3/6, 50%) were FISH-positive. Conclusions: The sensitivity and specificity of IHC was 100% and 95.8%, respectively. These data supported an IHC scoring algorithm in which ALK IHC scores of 0, 1, or 3 were highly compatible with FISH results, and IHC scores of 2 were variable. Based on these findings, the IHC assay using the 5A4 antibody reliably detected non-small cell lung cancer with ALK rearrangement and may be useful as a screening method to identify these tumors. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier Inc. | - |
dc.title | Screening of anaplastic lymphoma kinase rearrangement by immunohistochemistry in non-small cell lung cancer: Correlation with fluorescence in situ hybridization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1097/JTO.0b013e31820b82e8 | - |
dc.citation.journaltitle | Journal of Thoracic Oncology | - |
dc.identifier.wosid | 000287240100008 | - |
dc.identifier.scopusid | 2-s2.0-79951769488 | - |
dc.citation.endpage | 472 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 466 | - |
dc.citation.volume | 6 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Paik, Jin Ho | - |
dc.contributor.affiliatedAuthor | Choe, Gheeyoung | - |
dc.contributor.affiliatedAuthor | Lee, Choon-Taek | - |
dc.contributor.affiliatedAuthor | Lee, Jong Seok | - |
dc.contributor.affiliatedAuthor | Jheon, Sanghoon | - |
dc.contributor.affiliatedAuthor | Chung, Jin-Haeng | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | GROWTH-FACTOR-RECEPTOR | - |
dc.subject.keywordPlus | EML4-ALK FUSION GENE | - |
dc.subject.keywordPlus | ADENOCARCINOMA | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordPlus | FEATURES | - |
dc.subject.keywordPlus | RARE | - |
dc.subject.keywordAuthor | EML4-ALK | - |
dc.subject.keywordAuthor | Non-small cell lung cancer | - |
dc.subject.keywordAuthor | Immunohistochemistry | - |
dc.subject.keywordAuthor | Fluorescence in situ hybridization | - |
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