Publications
Detailed Information
The heat-shock protein 90 inhibitor, geldanamycin, induces apoptotic cell death in Epstein-Barr virus-positive NK/T-cell lymphoma by Akt down-regulation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jeon, Y. K. | - |
dc.contributor.author | Park, C. H. | - |
dc.contributor.author | Kim, K-Y | - |
dc.contributor.author | Li, Y. C. | - |
dc.contributor.author | Kim, J. | - |
dc.contributor.author | Kim, Y. A. | - |
dc.contributor.author | Paik, J-H | - |
dc.contributor.author | Park, B-K | - |
dc.contributor.author | Kim, C-W | - |
dc.contributor.author | Kim, Y-N | - |
dc.date.accessioned | 2023-05-08T08:27:30Z | - |
dc.date.available | 2023-05-08T08:27:30Z | - |
dc.date.created | 2023-05-04 | - |
dc.date.created | 2023-05-04 | - |
dc.date.issued | 2007-10 | - |
dc.identifier.citation | Journal of Pathology, Vol.213 No.2, pp.170-179 | - |
dc.identifier.issn | 0022-3417 | - |
dc.identifier.uri | https://hdl.handle.net/10371/192192 | - |
dc.description.abstract | NK/T-cell lymphoma (NKTL) is strongly associated with latent Epstein-Barr virus (EBV) infection. Recently, latent membrane protein 1 (LMP1), an EBV oncoprotein, was reported to activate the phosphatidylinositol-3 kinase (Pl3K)/Akt pathway for cell survival. Because geldanamycin (GA) and its derivative, 17-allylamino-17-demethoxygeldanamycin (17-AAG), exhibit anti-tumour activity by degrading HSP90 client proteins, including Akt, we investigated the effect of GA and 17-AAG on the survival of NKTL cell lines. EBV-positive NKTL cell lines, Hank-1 and NK-YS, and an EBV-negative NK leukaemia cell line, NK-L, were treated with PI3K and Akt inhibitors, GA, and 17-AAG, and were subjected to apoptosis and cell viability assays, and immunoblot analysis. EBV-positive B-lymphoblastoid cell lines IM9 and LMP1-transfected IM9 (IM9-LMP1) were also included. Hank-1 and NK-YS cell viability was compromised and apoptosis was induced by LY294002 (PI3K inhibitor) or Akt inhibitor II. GA or 17-AAG administration resulted in the apoptosis of NKTL cells, accompanied by Akt and pAkt down-regulation, caspase 3 activation, and mitochondrial membrane potential disruption. The intrinsic level of pAkt was higher in EBV-positive NKTL cells than in EBV-negative NK-L, and GA or 17-AAG decreased the viability of NKTL cells more efficiently than NK-L. Moreover, IM9-LMP1 was more sensitive to Akt inhibitor 11 or HSP90 inhibitors than IM9. Importantly, GA showed little effect on the viability of normal peripheral NK cells as non-neoplastic counterparts for comparison. In conclusion, this study suggests that the PI3K/Akt pathway is frequently activated in EBV-positive NKTL and that therapeutic modalities based on targeting the PI3K/Akt pathway with HSP90 inhibitors could be useful for achieving NKTL control. Copyright (c) 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. | - |
dc.language | 영어 | - |
dc.publisher | John Wiley & Sons Inc. | - |
dc.title | The heat-shock protein 90 inhibitor, geldanamycin, induces apoptotic cell death in Epstein-Barr virus-positive NK/T-cell lymphoma by Akt down-regulation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1002/path.2219 | - |
dc.citation.journaltitle | Journal of Pathology | - |
dc.identifier.wosid | 000250239100006 | - |
dc.identifier.scopusid | 2-s2.0-35349010666 | - |
dc.citation.endpage | 179 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 170 | - |
dc.citation.volume | 213 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Paik, J-H | - |
dc.contributor.affiliatedAuthor | Kim, C-W | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MEMBRANE-PROTEIN 1 | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE-AKT PATHWAY | - |
dc.subject.keywordPlus | HEAT-SHOCK PROTEIN-90 | - |
dc.subject.keywordPlus | HODGKINS-LYMPHOMA | - |
dc.subject.keywordPlus | GROWTH TRANSFORMATION | - |
dc.subject.keywordPlus | MULTIPLE-MYELOMA | - |
dc.subject.keywordPlus | MOLECULAR TARGET | - |
dc.subject.keywordPlus | LEUKEMIA-CELLS | - |
dc.subject.keywordPlus | CYCLE ARREST | - |
dc.subject.keywordPlus | HERBIMYCIN-A | - |
dc.subject.keywordAuthor | NK/T-cell lymphoma | - |
dc.subject.keywordAuthor | HSP90 inhibitor | - |
dc.subject.keywordAuthor | geldanamycin | - |
dc.subject.keywordAuthor | Epstein-Barr virus | - |
dc.subject.keywordAuthor | LMP1 | - |
dc.subject.keywordAuthor | akt | - |
dc.subject.keywordAuthor | p13-kinase | - |
dc.subject.keywordAuthor | apoptosis | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.