Publications
Detailed Information
Cord Blood Mesenchymal Stromal Cell-Conditioned Medium Protects Endothelial Cells via STAT3 Signaling
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bader, Andreas Matthaeus | - |
dc.contributor.author | Brodarac, Andreja | - |
dc.contributor.author | Klose, Kristin | - |
dc.contributor.author | Bieback, Karen | - |
dc.contributor.author | Choi, Yeong-Hoon | - |
dc.contributor.author | Kang, Kyung-Sun | - |
dc.contributor.author | Kurtz, Andreas | - |
dc.contributor.author | Stamm, Christof | - |
dc.date.accessioned | 2023-05-10T01:25:03Z | - |
dc.date.available | 2023-05-10T01:25:03Z | - |
dc.date.created | 2020-07-14 | - |
dc.date.issued | 2014-08 | - |
dc.identifier.citation | Cellular Physiology and Biochemistry, Vol.34 No.3, pp.646-657 | - |
dc.identifier.issn | 1015-8987 | - |
dc.identifier.uri | https://hdl.handle.net/10371/192309 | - |
dc.description.abstract | Background/Aims: Cell-based therapies may be useful for treating ischemic diseases, but the underlying mechanisms are incompletely understood. We investigated the impact of cord blood mesenchymal stromal cell (CBMSC)- or fibroblast (FB)-secreted factors on starved endothelial cells and determined the relevant intracellular signaling pathways. Methods: HUVECs were subjected to glucose/serum deprivation (GSD) in hypoxia or normoxia, in presence of CBMSC- or FB-conditioned medium (CM). Viability and proliferation were determined via WST-8 conversion and BrdU incorporation. Apoptosis was quantified by annexin V/ethidium homodimer-III staining, nuclear fragmentation and cell morphology. mRNA expression and protein phosphorylation were determined by real-time qPCR and western blot. Experiments were repeated in presence of small molecule inhibitors. Results: The negative impact of GSD was most pronounced at 21% O-2. Here, medium of CBMSCs and FBs increased viability and proliferation and reduced apoptosis of HUVECs. This was associated with increased STAT3 and ERK1/2 phosphorylation and BCL-2 expression. Under STAT3 inhibition, the beneficial effect of CBMSC-CM on viability and BCL-2 expression was abolished. Conclusion: Factors released by CBMSCs protect endothelial cells from the deleterious impact of GSD by activation of the STAT3 survival pathway. However, this phenomenon is not CBMSC-specific and can be reproduced using juvenile fibroblasts. Copyright (C) 2014 S. Karger AG, Basel | - |
dc.language | 영어 | - |
dc.publisher | S. Karger AG | - |
dc.title | Cord Blood Mesenchymal Stromal Cell-Conditioned Medium Protects Endothelial Cells via STAT3 Signaling | - |
dc.type | Article | - |
dc.identifier.doi | 10.1159/000363030 | - |
dc.citation.journaltitle | Cellular Physiology and Biochemistry | - |
dc.identifier.wosid | 000343765200004 | - |
dc.identifier.scopusid | 2-s2.0-84906609891 | - |
dc.citation.endpage | 657 | - |
dc.citation.number | 3 | - |
dc.citation.startpage | 646 | - |
dc.citation.volume | 34 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kang, Kyung-Sun | - |
dc.contributor.affiliatedAuthor | Kurtz, Andreas | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CRITICAL LIMB ISCHEMIA | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | MYOCARDIAL-INFARCTION | - |
dc.subject.keywordPlus | PARACRINE MECHANISMS | - |
dc.subject.keywordPlus | PROMOTE ANGIOGENESIS | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordAuthor | Cell therapy | - |
dc.subject.keywordAuthor | Endothelial cell | - |
dc.subject.keywordAuthor | Ischemia | - |
dc.subject.keywordAuthor | Stem cell | - |
dc.subject.keywordAuthor | Cord blood | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.