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Oblique-plane single-molecule localization microscopy for tissues and small intact animals
Cited 55 time in
Web of Science
Cited 60 time in Scopus
- Authors
- Issue Date
- 2019-09
- Publisher
- Nature Publishing Group
- Citation
- Nature Methods, Vol.16 No.9, pp.853-857
- Abstract
- Single-molecule localization microscopy (SMLM), while well established for cultured cells, is not yet fully compatible with tissue-scale samples. We introduce single-molecule oblique-plane microscopy (obSTORM), which by directly imaging oblique sections of samples with oblique light-sheet illumination offers a deep and volumetric SMLM platform that is convenient for standard tissue samples and small intact animals. We demonstrate super-resolution imaging at depths of up to 66 mu m for cells, Caenorhabditis elegans gonads, Drosophila melanogaster larval brain, mouse retina and brain sections, and whole stickleback fish.
- ISSN
- 1548-7091
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