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Preconditioning of canine adipose tissue-derived mesenchymal stem cells with deferoxamine potentiates anti-inflammatory effects by directing/reprogramming M2 macrophage polarization
DC Field | Value | Language |
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dc.contributor.author | Park, Su-Min | - |
dc.contributor.author | Li, Qiang | - |
dc.contributor.author | Ryu, Min-Ok | - |
dc.contributor.author | Nam, Aryung | - |
dc.contributor.author | An, Ju-Hyun | - |
dc.contributor.author | Yang, Ji-In | - |
dc.contributor.author | Kim, Sang-Min | - |
dc.contributor.author | Song, Woo-Jin | - |
dc.contributor.author | Youn, Hwa-Young | - |
dc.date.accessioned | 2023-06-27T06:36:34Z | - |
dc.date.available | 2023-06-27T06:36:34Z | - |
dc.date.created | 2020-03-04 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.citation | Veterinary Immunology and Immunopathology, Vol.219, p. 109973 | - |
dc.identifier.issn | 0165-2427 | - |
dc.identifier.uri | https://hdl.handle.net/10371/192872 | - |
dc.description.abstract | Preconditioning with hypoxia or hypoxia-mimetic agents has been tried with mesenchymal stem cells (MSCs) to improve the secretion of anti-inflammatory factors. These preconditioning procedures upregulate hypoxia inducible factor (HIF) 1-alpha leading to the transcription of HIF-dependent tissue protective and anti-inflammatory genes. Due to the limited number of studies exploring the activity of deferoxamine (DFO)-a hypoxia-mimetic agent in MSCs, we aimed to determine whether DFO can enhance the secretion of antiinflammatory substances in canine adipose tissue-derived (cAT)-MSCs. Furthermore, we investigated whether this activity of DFO could affect macrophage polarization and activate anti-inflammatory reactions. cAT-MSCs preconditioned with DFO exhibited enhanced secretion of anti-inflammatory factors such as prostaglandin E2 and tumor necrosis factor-alpha-stimulated gene-6. To evaluate the interaction between DFO preconditioned cAT-MSCs and macrophages, RAW 264.7 cells were co-cultured with cAT-MSCs using the Transwell system, and changes in the expression of factors related to macrophage polarization were analyzed using the quantitative real-time PCR and western blot assays. When RAW 264.7 cells were co-cultured with DFO preconditioned cAT-MSCs, the expression of M1 and M2 markers decreased and increased, respectively, compared to co-culturing with non-preconditioned cAT-MSCs. Thus, cAT-MSCs preconditioned with DFO can more effectively direct and reprogram macrophage polarization into the M2 phase, an anti-inflammatory state. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Preconditioning of canine adipose tissue-derived mesenchymal stem cells with deferoxamine potentiates anti-inflammatory effects by directing/reprogramming M2 macrophage polarization | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.vetimm.2019.109973 | - |
dc.citation.journaltitle | Veterinary Immunology and Immunopathology | - |
dc.identifier.wosid | 000509788500011 | - |
dc.identifier.scopusid | 2-s2.0-85075304535 | - |
dc.citation.startpage | 109973 | - |
dc.citation.volume | 219 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Youn, Hwa-Young | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | HYPOXIA-INDUCIBLE FACTORS | - |
dc.subject.keywordPlus | FACTOR-1-ALPHA STABILIZATION | - |
dc.subject.keywordPlus | MYOCARDIAL INJURY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | HIF-1 | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordAuthor | Anti-inflammatory | - |
dc.subject.keywordAuthor | Cell therapy | - |
dc.subject.keywordAuthor | Deferoxamine | - |
dc.subject.keywordAuthor | Hypoxia mimetic agent | - |
dc.subject.keywordAuthor | Mesenchymal stem cell | - |
dc.subject.keywordAuthor | Macrophage polarization | - |
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