Large-scale production of membrane proteins in saccharomyces cerevisiae: Using a green fluorescent protein fusion strategy in the production of membrane proteins

Abstract

The production of membrane proteins in the large quantities necessary for structural analysis requires many optimization steps. The GFP-fusion-based scheme described in earlier chapters ( Chapters 4 , 8 , and 16 ) facilitates these steps by allowing the selection of high yielding clones that produce detergent-stable membrane proteins. Here, we describe the experimental steps required to establish the reproducible, large-scale production and purification of membrane protein-GFP fusions using S. cerevisiae. © 2012 Springer Science+business Media, LLC.