Adhesive coacervate as a bioactive coating supplement for wound dressings

Abstract

Wound healing using growth factors is a clinically effective treatment method. However, it has a short biological half-life due to protease secreted into the wound area to break down the damaged extracellular matrix (ECM). The study shows a new coating supplement functionalizing adhesiveness using dopamine (DOPA) to the complex coacervate of fucoidan and poly-l-lysine (PLL), showing antioxidant properties that useful in wound healing. The dopamine-complex coacervate (D-Coa) was optimized to form maximum micro-droplets. The D-Coa, dopamine functionalized fucoidan, a marine-derived glycosaminoglycan, and poly-l-lysine (PLL) coacervates have shown promising protein delivery; sustained release profile, superior encapsulation ability, biocompatibility, and protection against harsh in-vivo environment, for tissue engineering applications as our previous fucoidan and poly-l-lysine (PLL) complex coacervate study was shown. Furthermore, the D-Coa was conducted superior adhesive forces and successfully coated onto collagen sponge homogeneously and rapidly. From in vivo performance tests using at skin defect models, our D-Coa coating patch were demonstrated successful wound regeneration. This game changer coating supplements can rescue biological half-life and reduce coating time, preventing limited application of scaffold such as commercially available collagen sponge.