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Toxicity Assessment of Transfluthrin, Benzyl Butyl Phthalate, and 17β-Estradiol on the Primary Fibroblast of the Striped Field Mouse, Apodemus agrarius : Toxicity Assessment of Transfluthrin, Benzyl Butyl Phthalate, and 17 beta-Estradiol on the Primary Fibroblast of the Striped Field Mouse, Apodemus agrarius

Cited 1 time in Web of Science Cited 1 time in Scopus
Authors

Lee, Ji Min; Kim, Ukjin; Lee, Byoung-Hee; Chang, Seo-Na; Song, Juha; Ryu, Bokyeong; Park, Jae-Hak

Issue Date
2021-01
Publisher
Begell House
Citation
Journal of Environmental Pathology, Toxicology and Oncology, Vol.40 No.2, pp.65-79
Abstract
Environmental pollution (EP) is a well-known threat to wild animals, but its toxicological impact is poorly understood. In vitro toxicity evaluation using cells of lower predators could be a promising way to assess and monitor the effects of EPs on whole wildlife populations that are related in the food web. Here, we describe EPs' toxic effect and mechanism in the primary fibroblast derived from the embryo of the striped field mouse, Apodemus agrarius. Characterization of the primary fibroblast was via morphology, genetics, immunocytochernistiy, and stable culture conditions for optimal toxicity screening. Cell viability assays-MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and lactate dehydrogenase (LDH)-were performed to observe cytotoxicity, and quantitative PCR was conducted to confirm gene alteration by EP exposure. MTT and LDH assays confirmed the cytotoxicity of transfluthrin (TF), benzyl butyl phthalate (TIRP), and 17 beta-estradiol (E2) with IC50 values of 10.56 mu M, 10.82 mu M, and 24.08 mu M, respectively, following 48-h exposures. mRNA expression of androgen-binding protein, growth hormone receptor, cytochrome C oxidase, and cytochmme P450-1A1 was induced after exposure to TF, BBP, and E2. We unveiled new EP mechanisms at the mammalian cellular level and discovered potential biomarker genes for monitoring of EPs. Based on our findings, we propose the primary fibroblast of A. agrarius as a valuable model to assess the toxicological effects of EP on wildlife.
ISSN
0731-8898
URI
https://hdl.handle.net/10371/194740
DOI
https://doi.org/10.1615/JEnvironPatholToxicolOncol.2021036845
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  • College of Veterinary Medicine
  • Department of Veterinary Medicine
Research Area Laboratory Animal Medicine, Toxicologic Pathology

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