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Essential role of toll-like receptor 4 in Acinetobacter baumannii-induced immune responses in immune cells
DC Field | Value | Language |
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dc.contributor.author | Kim, Chang-Hwan | - |
dc.contributor.author | Jeong, Yu-Jin | - |
dc.contributor.author | Lee, Junglim | - |
dc.contributor.author | Jeon, Soo-Jin | - |
dc.contributor.author | Park, Se-Ra | - |
dc.contributor.author | Kang, Min-Jung | - |
dc.contributor.author | Park, Jae-Hak | - |
dc.contributor.author | Park, Jong-Hwan | - |
dc.date.accessioned | 2023-07-07T08:00:42Z | - |
dc.date.available | 2023-07-07T08:00:42Z | - |
dc.date.created | 2020-07-17 | - |
dc.date.created | 2020-07-17 | - |
dc.date.created | 2020-07-17 | - |
dc.date.issued | 2013-01 | - |
dc.identifier.citation | Microbial Pathogenesis, Vol.54 No.1, pp.20-25 | - |
dc.identifier.issn | 0882-4010 | - |
dc.identifier.uri | https://hdl.handle.net/10371/194802 | - |
dc.description.abstract | TLR4 is a membrane sensor for lipopolysaccharide (LPS), a major cell wall component of gram-negative bacteria. In this study, we investigated the role of TLR4 on innate immune responses in immune cells against Acinetobacter baumannii. Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were isolated from WT and TLR4-deficient mice and infected with A. baumannii ATCC 15150. ELISA assay revealed that the production of IL-6 and TNF-alpha by A. baumannii was impaired in TLR4-deficient macrophages. However, absence of TLR2 did not affect A. baumannii-induced cytokines production in BMDMs. In addition, TLR4 was required for the optimal production of IL-6, TNF-alpha, and IL-12 in BMDCs in response to A. baumannii. Western blot analysis showed that A. baumannii leads to the activation of NF-kappa B and MAPKs (p38, ERIK, and JNK) in macrophages via TLR4-dependent pathway. mRNA expression of iNOS and NO production was elicited in WT BMDMs in response to A. baumannii, which was abolished in TLR4-deficienct cells. Bacterial killing ability against A. baumannii was impaired in TLR4-deficient BMDMs. In addition, A. baumannii induced apoptosis in BMDMs via TLR4-independent pathway. Our results demonstrate that TLR4 is essential for initiating innate immune response of macrophages against A. baumannii infection. (C) 2012 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Essential role of toll-like receptor 4 in Acinetobacter baumannii-induced immune responses in immune cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.micpath.2012.08.008 | - |
dc.citation.journaltitle | Microbial Pathogenesis | - |
dc.identifier.wosid | 000314134700002 | - |
dc.identifier.scopusid | 2-s2.0-84871172478 | - |
dc.citation.endpage | 25 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 20 | - |
dc.citation.volume | 54 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Jae-Hak | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PATHOGEN RECOGNITION | - |
dc.subject.keywordPlus | TOLL-LIKE-RECEPTOR-4 | - |
dc.subject.keywordPlus | MACROPHAGES | - |
dc.subject.keywordPlus | LIPOPOLYSACCHARIDE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | RLRS | - |
dc.subject.keywordPlus | NLRS | - |
dc.subject.keywordPlus | TLRS | - |
dc.subject.keywordAuthor | Acinetobacter baumannii | - |
dc.subject.keywordAuthor | TLR4 | - |
dc.subject.keywordAuthor | Immune response | - |
dc.subject.keywordAuthor | Cytokines | - |
dc.subject.keywordAuthor | Nitric oxide | - |
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