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Induction of systemic immunity through nasal-associated lymphoid tissue (NALT) of mice intranasally immunized with Brucella abortus malate dehydrogenase-loaded chitosan nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Shim, Soojin | - |
dc.contributor.author | Soh, Sang Hee | - |
dc.contributor.author | Im, Young Bin | - |
dc.contributor.author | Ahn, Choonghyun | - |
dc.contributor.author | Park, Hong-Tae | - |
dc.contributor.author | Park, Hyun-Eui | - |
dc.contributor.author | Park, Woo Bin | - |
dc.contributor.author | Kim, Suji | - |
dc.contributor.author | Yoo, Han Sang | - |
dc.date.accessioned | 2023-10-30T02:02:42Z | - |
dc.date.available | 2023-10-30T02:02:42Z | - |
dc.date.created | 2020-06-15 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.citation | PLoS ONE, Vol.15 No.2 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10371/196011 | - |
dc.description.abstract | Infection with Brucella abortus causes contagious zoonosis, brucellosis, and leads to abortion in animals and chronic illness in humans. Chitosan nanoparticles (CNs), biocompatible and nontoxic polymers, acts as a mucosal adjuvant. In our previous study, B. abortus malate dehydrogenase (Mdh) was loaded in CNs, and it induced high production of pro-inflammatory cytokines in THP-1 cells and systemic IgA in BALB/C mice. In this study, the time-series gene expression analysis of nasal-associated lymphoid tissue (NALT) was performed to identify the mechanism by which Mdh affect the target site of nasal immunization. We showed that intranasal immunization of CNs-Mdh reduced cell viability of epithelial cells and muscle cells at first 1 h, then induced cellular movement of immune cells such as granulocytes, neutrophils and lymphocytes at 6h, and activated IL-6 signaling pathway at 12h within NALT. These activation of immune cells also promoted signaling pathway for high-mobility group box 1 protein (HMGB1), followed by the maturation of DCs required for mucosal immunity. The CNs also triggered the response to other organism and inflammatory response, showing it is immune-enhancing adjuvant. The ELISA showed that significant production of specific IgA was detected in the fecal excretions and genital secretions from the CNs-Mdh-immunized group after 2 weeks-post immunization. Collectively, these results suggest that B. abortus Mdh-loaded CNs triggers activation of HMGB1, IL-6 and DCs maturation signaling within NALT and induce production of systemic IgG and IgA. | - |
dc.language | 영어 | - |
dc.publisher | Public Library of Science | - |
dc.title | Induction of systemic immunity through nasal-associated lymphoid tissue (NALT) of mice intranasally immunized with Brucella abortus malate dehydrogenase-loaded chitosan nanoparticles | - |
dc.type | Article | - |
dc.identifier.doi | 10.1371/journal.pone.0228463 | - |
dc.citation.journaltitle | PLoS ONE | - |
dc.identifier.wosid | 000534623800039 | - |
dc.identifier.scopusid | 2-s2.0-85079083932 | - |
dc.citation.number | 2 | - |
dc.citation.volume | 15 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Yoo, Han Sang | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | PATTERN | - |
dc.subject.keywordPlus | VIVO | - |
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