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High-resolution open-top axially swept light sheet microscopy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Soohyun | - |
dc.contributor.author | Na, Myeongsu | - |
dc.contributor.author | Chang, Sunghoe | - |
dc.contributor.author | Kim, Ki Hean | - |
dc.date.accessioned | 2023-12-05T04:06:50Z | - |
dc.date.available | 2023-12-05T13:07:38Z | - |
dc.date.issued | 2023-11-08 | - |
dc.identifier.citation | BMC Biology, Vol.21(1):248 | ko_KR |
dc.identifier.issn | 1741-7007 | - |
dc.identifier.uri | https://hdl.handle.net/10371/197594 | - |
dc.description.abstract | Background
Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for the high-throughput cellular imaging of optically cleared, large-sized specimens, such as the brain. Despite the development of various OT-LSM techniques, achieving submicron resolution in all dimensions remains. Results We developed a high-resolution open-top axially swept LSM (HR-OTAS-LSM) for high-throughput and high-resolution imaging in all dimensions. High axial and lateral resolutions were achieved by using an aberration-corrected axially swept excitation light sheet in the illumination arm and a high numerical aperture (NA) immersion objective lens in the imaging arm, respectively. The high-resolution, high-throughput visualization of neuronal networks in mouse brain and retina specimens validated the performance of HR-OTAS-LSM. Conclusions The proposed HR-OTAS-LSM method represents a significant advancement in the high-resolution mapping of cellular networks in biological systems such as the brain and retina. | ko_KR |
dc.description.sponsorship | This work was supported in part by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2020R1A2C3009309, RS-2023–00219421) and by Samsung Research Funding & Incubation Center of Samsung Electronics under Project Number SRFC-IT2101-05. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BMC | ko_KR |
dc.subject | Light sheet microscopy | - |
dc.subject | Open-top | - |
dc.subject | Axial swept | - |
dc.subject | Aberration correction | - |
dc.subject | Optically cleared tissue | - |
dc.title | High-resolution open-top axially swept light sheet microscopy | ko_KR |
dc.type | Article | ko_KR |
dc.identifier.doi | 10.1186/s12915-023-01747-3 | ko_KR |
dc.citation.journaltitle | BMC Biology | ko_KR |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2023-11-12T04:10:36Z | - |
dc.citation.number | 1 | ko_KR |
dc.citation.volume | 21 | ko_KR |
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