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Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis
DC Field | Value | Language |
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dc.contributor.author | Quyen Thi Nguyen | - |
dc.contributor.author | Yang, Jihyun | - |
dc.contributor.author | Byun, Jae-Won | - |
dc.contributor.author | Pyo, Hyun Mi | - |
dc.contributor.author | Park, Mi-Young | - |
dc.contributor.author | Ku, Bok Kyung | - |
dc.contributor.author | Nah, Jinju | - |
dc.contributor.author | Ryoo, Soyoon | - |
dc.contributor.author | Wee, Sung-Hwan | - |
dc.contributor.author | Choi, Kang-Seuk | - |
dc.contributor.author | Poo, Haryoung | - |
dc.date.accessioned | 2023-12-11T04:12:54Z | - |
dc.date.available | 2023-12-11T04:12:54Z | - |
dc.date.created | 2020-03-04 | - |
dc.date.issued | 2019-12 | - |
dc.identifier.citation | Pathogens, Vol.8 No.4, p. 301 | - |
dc.identifier.issn | 2076-0817 | - |
dc.identifier.uri | https://hdl.handle.net/10371/198123 | - |
dc.description.abstract | Foot-and-mouth disease (FMD) is a highly contagious and economically devastating disease affecting cloven-hoofed livestock worldwide. FMD virus (FMDV) type A is one of the most common causes of FMD outbreaks among the seven FMDV serotypes, and its serological diagnosis is therefore important to confirm FMDV type A infection and to determine FMD vaccine efficacy. Here, we generated monoclonal antibodies (mAbs) specific to FMDV type A via hybridoma systems using an inactivated FMDV type A (A22/Iraq/1964) and found 4 monoclones (#29, #106, #108, and #109) with high binding reactivity to FMDV type A among 594 primary clones. In particular, the #106 mAb had a higher binding reactivity to the inactivated FMDV type A than the other mAbs and a commercial mAb. Moreover, the #106 mAb showed no cross-reactivity to inactivated FMDV type South African territories 1, 2, and 3, and low reactivity to inactivated FMDV type O (O-1 Manisa). Importantly, the solid-phase competitive ELISA (SPCE) using horseradish peroxidase (HRP)-conjugated #106 mAb detected FMDV type A-specific Abs in sera from FMD type A-vaccinated cattle more effectively than a commercial SPCE. These results suggest that the newly developed FMDV type A-specific mAb might be useful for diagnostic approaches for detecting Abs against FMDV type A. | - |
dc.language | 영어 | - |
dc.publisher | MDPI AG | - |
dc.title | Development of Monoclonal Antibody Specific to Foot-and-Mouth Disease Virus Type A for Serodiagnosis | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/pathogens8040301 | - |
dc.citation.journaltitle | Pathogens | - |
dc.identifier.wosid | 000506652300144 | - |
dc.identifier.scopusid | 2-s2.0-85077206715 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 301 | - |
dc.citation.volume | 8 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Choi, Kang-Seuk | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PHASE COMPETITION ELISA | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | EMERGENCE | - |
dc.subject.keywordPlus | IMPACTS | - |
dc.subject.keywordAuthor | foot-and-mouth disease | - |
dc.subject.keywordAuthor | type A | - |
dc.subject.keywordAuthor | structural protein | - |
dc.subject.keywordAuthor | monoclonal antibody | - |
dc.subject.keywordAuthor | solid-phase competitive ELISA | - |
dc.subject.keywordAuthor | sensitivity | - |
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