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Disruption of G0/G1 switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken : Disruption of G(0)/G(1) switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken
DC Field | Value | Language |
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dc.contributor.author | Park, Tae Sub | - |
dc.contributor.author | Park, Joonghoon | - |
dc.contributor.author | Lee, Jeong Hyo | - |
dc.contributor.author | Park, Jeong-Woong | - |
dc.contributor.author | Park, Byung-Chul | - |
dc.date.accessioned | 2023-12-11T05:46:42Z | - |
dc.date.available | 2023-12-11T05:46:42Z | - |
dc.date.created | 2019-08-28 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.citation | FASEB Journal, Vol.33 No.1, pp.1188-1198 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://hdl.handle.net/10371/198311 | - |
dc.description.abstract | Chicken as a food source is one of the most widespread domestic animals, and it has been used extensively as a research model. The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system is the most efficient and reliable tool for precise genome-targeted modification and has generated considerable excitement for industrial applications, as well as biologic science. Unlike in mammals, germline-transmittable primordial germ cells (PGCs) in chicken were used as an alternative strategy for the production of genetically altered chickens. Here, by combining the CRISPR-Cas9 platform and germ cell-mediated germline transmission, we generated G(0)/G(1) switch gene 2 (G0S2) knockout (KO) chickens, and G0S2 null KO chickens showed a dramatic reduction of abdominal fat deposition without affecting other economic traits. Additionally, G0S2 null KO chickens had altered fatty acid compositions in their blood and abdominal fat compared with wild-type chickens under normal dietary conditions. The global mRNA sequencing data showed that G0S2 disruption in chickens would activate the adipose tissue-specific peroxisomal oxidation pathway, and enoyl-coenzyme A (CoA), hydratase/3-hydroxyacyl CoA dehydrogenase might be a target molecule in metabolic homeostasis in the chicken adipose tissue. Our results demonstrate that the CRISPR-Cas9 system with chicken PGCs can facilitate the production of specific genome-edited chickens for practical applications, as well as basic research.Park, T. S., Park, J., Lee, J. H., Park, J.-W., Park, B.-C. Disruption of G(0)/G(1) switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken. | - |
dc.language | 영어 | - |
dc.publisher | Federation of American Societies for Experimental Biology | - |
dc.title | Disruption of G0/G1 switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken | - |
dc.title.alternative | Disruption of G(0)/G(1) switch gene 2 (G0S2) reduced abdominal fat deposition and altered fatty acid composition in chicken | - |
dc.type | Article | - |
dc.identifier.doi | 10.1096/fj.201800784R | - |
dc.citation.journaltitle | FASEB Journal | - |
dc.identifier.wosid | 000457401500094 | - |
dc.identifier.scopusid | 2-s2.0-85059238144 | - |
dc.citation.endpage | 1198 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 1188 | - |
dc.citation.volume | 33 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Tae Sub | - |
dc.contributor.affiliatedAuthor | Park, Byung-Chul | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ADIPOSE LIPOLYSIS | - |
dc.subject.keywordPlus | ENERGY-METABOLISM | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | LIVER | - |
dc.subject.keywordPlus | DIET | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | Cas9 nuclease | - |
dc.subject.keywordAuthor | knockout | - |
dc.subject.keywordAuthor | primordial germ cell | - |
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