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Modification of PCNA by ISG15 Plays a Crucial Role in Termination of Error-Prone Translesion DNA Synthesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jung Mi | - |
dc.contributor.author | Yang, Seung Wook | - |
dc.contributor.author | Yu, Kyung Ryun | - |
dc.contributor.author | Ka, Seung Hyun | - |
dc.contributor.author | Lee, Seong Won | - |
dc.contributor.author | Seol, Jae Hong | - |
dc.contributor.author | Jeon, Young Joo | - |
dc.contributor.author | Chung, Chin Ha | - |
dc.date.accessioned | 2023-12-11T07:38:00Z | - |
dc.date.available | 2023-12-11T07:38:00Z | - |
dc.date.created | 2020-08-19 | - |
dc.date.created | 2020-08-19 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.citation | Molecular Cell, Vol.54 No.4, pp.626-638 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | https://hdl.handle.net/10371/198553 | - |
dc.description.abstract | In response to DNA damage, PCNA is mono-ubiquitinated and triggers translesion DNA synthesis (TLS) by recruiting polymerase-eta. However, it remained unknown how error-prone TLS is turned off after DNA lesion bypass to prevent mutagenesis. Here we showed that ISG15 modification (ISGylation) of PCNA plays a key role in TLS termination. Upon UV irradiation, EFP, an ISG15 E3 ligase, bound to mono-ubiquitinated PCNA and promoted its ISGylation. ISGylated PCNA then tethered USP10 for deubiquitination and in turn the release of polymerase-h from PCNA. Eventually, PCNA was deISGylated by UBP43 for reloading of replicative DNA polymerases and resuming normal DNA replication. However, ISGylation-defective Lys-to-Arg mutations in PCNA or knockdown of any of ISG15, EFP, or USP10 led to persistent recruitment of mono-ubiquitinated PCNA and polymerase-eta to nuclear foci, causing an increase in mutation frequency. These findings establish a crucial role of PCNA ISGylation in termination of error-prone TLS for preventing excessive mutagenesis. | - |
dc.language | 영어 | - |
dc.publisher | Cell Press | - |
dc.title | Modification of PCNA by ISG15 Plays a Crucial Role in Termination of Error-Prone Translesion DNA Synthesis | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.molcel.2014.03.031 | - |
dc.citation.journaltitle | Molecular Cell | - |
dc.identifier.wosid | 000336380900010 | - |
dc.identifier.scopusid | 2-s2.0-84901228683 | - |
dc.citation.endpage | 638 | - |
dc.citation.number | 4 | - |
dc.citation.startpage | 626 | - |
dc.citation.volume | 54 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Seol, Jae Hong | - |
dc.contributor.affiliatedAuthor | Chung, Chin Ha | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | UBIQUITIN-LIKE PROTEIN | - |
dc.subject.keywordPlus | POLYMERASE-ETA | - |
dc.subject.keywordPlus | MONOUBIQUITINATED PCNA | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | SUMO | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | CONJUGATION | - |
dc.subject.keywordPlus | ENZYME | - |
dc.subject.keywordPlus | REPAIR | - |
dc.subject.keywordPlus | RECOGNITION | - |
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