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Effects of G and SH Truncation on the Replication, Virulence, and Immunogenicity of Avian Metapneumovirus
DC Field | Value | Language |
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dc.contributor.author | Hong, Seung-Min | - |
dc.contributor.author | Ha, Eun-Jin | - |
dc.contributor.author | Kim, Ho-Won | - |
dc.contributor.author | Kim, Seung-Ji | - |
dc.contributor.author | Ahn, Sun-Min | - |
dc.contributor.author | An, Se-Hee | - |
dc.contributor.author | Kim, Gun | - |
dc.contributor.author | Kim, Suji | - |
dc.contributor.author | Kwon, Hyuk-Joon | - |
dc.contributor.author | Choi, Kang-Seuk | - |
dc.date.accessioned | 2024-02-15T04:13:58Z | - |
dc.date.available | 2024-02-15T04:13:58Z | - |
dc.date.created | 2024-02-08 | - |
dc.date.issued | 2024-01 | - |
dc.identifier.citation | Vaccines, Vol.12 No.1, p. 106 | - |
dc.identifier.issn | 2076-393X | - |
dc.identifier.uri | https://hdl.handle.net/10371/198996 | - |
dc.description.abstract | Four mutants varying the length of the G and SH genes, including a G-truncated mutant (Delta G) and three G/SH-truncated mutants (Delta SH/G-1, Delta SH/G-2, and Delta SH/G-3), were generated via serially passaging the avian metapneumovirus strain SNU21004 into the cell lines Vero E6 and DF-1 and into embryonated chicken eggs. The mutant Delta G particles resembled parental virus particles except for the variance in the density of their surface projections. G and G/SH truncation significantly affected the viral replication in chickens' tracheal ring culture and in infected chickens but not in the Vero E6 cells. In experimentally infected chickens, mutant Delta G resulted in the restriction of viral replication and the attenuation of the virulence. The mutants Delta G and Delta SH/G-1 upregulated three interleukins (IL-6, IL-12, and IL-18) and three interferons (IFN alpha, IFN beta, and IFN gamma) in infected chickens. In addition, the expression levels of innate immunity-related genes such as Mda5, Rig-I, and Lgp2, in BALB/c mice were also upregulated when compared to the parental virus. Immunologically, the mutant Delta G induced a strong, delayed humoral immune response, while the mutant Delta SH/G-1 induced no humoral immune response. Our findings indicate the potential of the mutant Delta G but not the mutant Delta SH/G-1 as a live attenuated vaccine candidate. | - |
dc.language | 영어 | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Effects of G and SH Truncation on the Replication, Virulence, and Immunogenicity of Avian Metapneumovirus | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/vaccines12010106 | - |
dc.citation.journaltitle | Vaccines | - |
dc.identifier.wosid | 001151142800001 | - |
dc.identifier.scopusid | 2-s2.0-85183087825 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 106 | - |
dc.citation.volume | 12 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Kwon, Hyuk-Joon | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | ATTACHMENT G-GLYCOPROTEIN | - |
dc.subject.keywordPlus | SMALL HYDROPHOBIC SH | - |
dc.subject.keywordPlus | SUBTYPE-C | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | DELETION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordAuthor | avian metapneumovirus | - |
dc.subject.keywordAuthor | G and SH truncation | - |
dc.subject.keywordAuthor | virus replication | - |
dc.subject.keywordAuthor | attenuation | - |
dc.subject.keywordAuthor | immunogenicity | - |
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