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Hypoxia-alleviating hemoglobin nanoclusters for sensitizing chemo-photodynamic therapy of cervical cancer
DC Field | Value | Language |
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dc.contributor.author | Lee, Han Sol | - |
dc.contributor.author | Yoo, So-Yeol | - |
dc.contributor.author | Lee, Sang Min | - |
dc.contributor.author | Kang, Nae-Won | - |
dc.contributor.author | Kim, Sang Kyum | - |
dc.contributor.author | Song, Gyu Yong | - |
dc.contributor.author | Kim, Dae-Duk | - |
dc.contributor.author | Lee, Jae-Young | - |
dc.date.accessioned | 2024-04-26T00:23:53Z | - |
dc.date.available | 2024-04-26T00:23:53Z | - |
dc.date.created | 2023-02-15 | - |
dc.date.issued | 2023-02 | - |
dc.identifier.citation | Chemical Engineering Journal, Vol.457 | - |
dc.identifier.issn | 1385-8947 | - |
dc.identifier.uri | https://hdl.handle.net/10371/199461 | - |
dc.description.abstract | © 2022 The Author(s)Hypoxia-alleviating hemoglobin (Hb) nanoclusters (NCs) have been developed for sensitizing combined chemo-photodynamic therapy (chemo-PDT) of cervical cancer. Hb was conjugated with chlorin e6 and biotinylated polyethylene glycol and adsorbed with doxorubicin (DOX), resulting in the self-assembly of 220 nm diameter protein NCs. These oxygen-carrying NCs, namely DOX@HPBC, exhibited improved colloidal stability in the serum compared to that of native Hb and pH-responsive drug release favorable for cancer treatment. DOX@HPBC alleviated hypoxia by 64.8% in HeLa cells cultured under hypoxia and normalized the levels of related biomarkers, HIF-1α and MDR1, with enhanced cellular uptake via biotin–receptor interactions. Moreover, antitumor efficacy tests performed in HeLa monolayer and spheroid cultures revealed that DOX@HPBC possessed 3.8-fold improved therapeutic efficacy compared to DOX and chlorin e6 physical mixture, showing synergism between chemotherapy and PDT. DOX@HPBC-mediated chemo-PDT significantly suppressed tumor growth in heterotopic (96.6% reduction in tumor volume compared to no-intervention) and orthotopic (comparable uterus weight to the normal group) HeLa-xenografted mice with no significant toxicity. These results showed the hypoxia amelioration effect, which lowered HIF-1α and MDR1 levels in tumor tissues, and efficient DOX distribution to hypoxic regions of the tumor, suggesting that DOX@HPBC could be a promising chemo-PDT strategy for cervical cancer treatment. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier B.V. | - |
dc.title | Hypoxia-alleviating hemoglobin nanoclusters for sensitizing chemo-photodynamic therapy of cervical cancer | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.cej.2022.141224 | - |
dc.citation.journaltitle | Chemical Engineering Journal | - |
dc.identifier.wosid | 000921139900001 | - |
dc.identifier.scopusid | 2-s2.0-85145297079 | - |
dc.citation.volume | 457 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Lee, Jae-Young | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | CHONDROITIN SULFATE | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | TUMOR | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordPlus | ENHANCE | - |
dc.subject.keywordPlus | CARRIER | - |
dc.subject.keywordPlus | PEG | - |
dc.subject.keywordAuthor | Cervical cancer | - |
dc.subject.keywordAuthor | Chemo-photodynamic therapy | - |
dc.subject.keywordAuthor | Hemoglobin | - |
dc.subject.keywordAuthor | Hypoxia alleviation | - |
dc.subject.keywordAuthor | Protein nanoclusters | - |
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