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Development of HPLC method for the determination of buspirone in rat plasma using fluorescence detection and its application to a pharmacokinetic study
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Ki Taek | - |
dc.contributor.author | Lee, Jae-Young | - |
dc.contributor.author | Park, Ju-Hwan | - |
dc.contributor.author | Kim, Min Hwan | - |
dc.contributor.author | Kim, Ji-Su | - |
dc.contributor.author | Shin, Hyeon-Jong | - |
dc.contributor.author | Kang, Naewon | - |
dc.contributor.author | Cho, Hyun-Jong | - |
dc.contributor.author | Yoon, In-Soo | - |
dc.contributor.author | Kim, Dae-Duk | - |
dc.date.accessioned | 2024-04-26T00:26:29Z | - |
dc.date.available | 2024-04-26T00:26:29Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2016-11 | - |
dc.identifier.citation | Chemical and Pharmaceutical Bulletin, Vol.64 No.11, pp.1582-1588 | - |
dc.identifier.issn | 0009-2363 | - |
dc.identifier.uri | https://hdl.handle.net/10371/199507 | - |
dc.description.abstract | A simple and sensitive analytical method for the quantitative determination of buspirone in rat plasma by HPLC with fluorescence detection was developed and validated using naproxen as an internal standard. A relatively small-volume (150 mu L) aliquot of rat plasma sample was prepared by a simple deproteinization procedure using acetonitrile as a precipitating organic solvent. Chromatographic separation was performed using Kinetex(R) C8 column with an isocratic mobile phase consisting of acetonitrile and 10-mM potassium phosphate buffer (pH 6.0) at a flow rate of 1.0 mL/min. The eluent was monitored by fluorescence detector at a wavelength pair of 237/380 nm (excitation/emission). The linearity was established at 20.0-5000 ng/mL, and the limit of detection was 6.51 ng/mL. The precision (<= 14.6%), accuracy (89.2-108%), and stability (89.1-101%) were within acceptable ranges. The newly developed method was successfully applied to intravenous and oral pharmacokinetic studies of buspirone in rats. | - |
dc.language | 영어 | - |
dc.publisher | Pharmaceutical Society of Japan | - |
dc.title | Development of HPLC method for the determination of buspirone in rat plasma using fluorescence detection and its application to a pharmacokinetic study | - |
dc.type | Article | - |
dc.identifier.doi | 10.1248/cpb.c16-00405 | - |
dc.citation.journaltitle | Chemical and Pharmaceutical Bulletin | - |
dc.identifier.wosid | 000386745100008 | - |
dc.identifier.scopusid | 2-s2.0-84993993654 | - |
dc.citation.endpage | 1588 | - |
dc.citation.number | 11 | - |
dc.citation.startpage | 1582 | - |
dc.citation.volume | 64 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Jae-Young | - |
dc.contributor.affiliatedAuthor | Kim, Dae-Duk | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | TANDEM MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | DRUG | - |
dc.subject.keywordPlus | QUANTITATION | - |
dc.subject.keywordPlus | FUTURE | - |
dc.subject.keywordAuthor | buspirone | - |
dc.subject.keywordAuthor | HPLC | - |
dc.subject.keywordAuthor | fluorescence detection | - |
dc.subject.keywordAuthor | rat plasma | - |
dc.subject.keywordAuthor | pharmacokinetics | - |
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