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Determination of manassantin B in rat plasma using a high performance liquid chromatography with fluorescence detection and its quantitative application to pharmacokinetic study
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae-Young | - |
dc.contributor.author | Song, Jae-Hyoung | - |
dc.contributor.author | Yoon, In-Soo | - |
dc.contributor.author | Ko, Hyun-Jeong | - |
dc.contributor.author | Kim, Dae-Duk | - |
dc.contributor.author | Cho, Hyun-Jong | - |
dc.date.accessioned | 2024-04-26T00:27:03Z | - |
dc.date.available | 2024-04-26T00:27:03Z | - |
dc.date.created | 2017-11-15 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol.1011, pp.121-127 | - |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.uri | https://hdl.handle.net/10371/199516 | - |
dc.description.abstract | A simple, sensitive, rapid, and reproducible analytical method of manassantin B in rat plasma by high performance liquid chromatography with fluorescence detection (HPLC-FL) was developed for its application to pharmacokinetic study in rats. Valsartan (VST) was used as an internal standard (IS) in this quantitative analytical method. Manassantin B and VST were extracted by simple and efficient protein precipitation method. Manassantin B was detected at 282/322 nm (excitation/emission) wavelengths using FL detector. The chromatographic separation was obtained with reverse phase C18 column and the mobile phase composed of potassium phosphate buffer containing 0.025% trifluoroacetic acid (pH 2.5; 5 mM) and acetonitrile including 0.025% trifluoroacetic acid (20:80, v/v) at 1.0 mL/min flow rate. The linearity was established at 25.0-10000 ng/mL and the lower limit of detection (LLOD) was 7 ng/mL. The intra- and inter-day accuracy and precision values of manassantin B were within +/- 15% of the theroretical values and <9% from the nominal concentrations, respectively. Accuracy and precision values of manassantin B after stability tests were also within the acceptable ranges. Developed assay was also successfully applied to pharmacokinetic study after intravenous administration of manassantin B in rats. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.publisher | Elsevier BV | - |
dc.title | Determination of manassantin B in rat plasma using a high performance liquid chromatography with fluorescence detection and its quantitative application to pharmacokinetic study | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.jchromb.2015.12.058 | - |
dc.citation.journaltitle | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | - |
dc.identifier.wosid | 000369458600016 | - |
dc.identifier.scopusid | 2-s2.0-84953372239 | - |
dc.citation.endpage | 127 | - |
dc.citation.startpage | 121 | - |
dc.citation.volume | 1011 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Lee, Jae-Young | - |
dc.contributor.affiliatedAuthor | Kim, Dae-Duk | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SAURURUS-CHINENSIS | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | LIGNANS | - |
dc.subject.keywordPlus | ROOTS | - |
dc.subject.keywordAuthor | Manassantin B | - |
dc.subject.keywordAuthor | Validation | - |
dc.subject.keywordAuthor | HPLC | - |
dc.subject.keywordAuthor | Fluorescence detection | - |
dc.subject.keywordAuthor | Pharmacokinetics | - |
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