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Naegleria fowleri Extracellular Vesicles Induce Proinflammatory Immune Responses in BV-2 Microglial Cells
DC Field | Value | Language |
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dc.contributor.author | Le, Huong Giang | - |
dc.contributor.author | Kang, Jung-Mi | - |
dc.contributor.author | Vo, Tuan Cuong | - |
dc.contributor.author | Yoo, Won Gi | - |
dc.contributor.author | Na, Byoung-Kuk | - |
dc.date.accessioned | 2024-05-02T05:35:56Z | - |
dc.date.available | 2024-05-02T05:35:56Z | - |
dc.date.created | 2024-04-29 | - |
dc.date.created | 2024-04-29 | - |
dc.date.issued | 2023-09 | - |
dc.identifier.citation | International Journal of Molecular Sciences, Vol.24 No.17, p. 13623 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://hdl.handle.net/10371/200375 | - |
dc.description.abstract | Extracellular vesicles (EVs) of protozoan parasites have diverse biological functions that are essential for parasite survival and host-parasite interactions. In this study, we characterized the functional properties of EVs from Naegleria fowleri, a pathogenic amoeba that causes a fatal brain infection called primary amoebic meningoencephalitis (PAM). N. fowleri EVs (NfEVs) have been shown to be internalized by host cells such as C6 glial cells and BV-2 microglial cells without causing direct cell death, indicating their potential roles in modulating host cell functions. NfEVs induced increased expression of proinflammatory cytokines and chemokines such as TNF-a, IL-1a, IL-1 beta, IL-6, IL-17, IFN-?, MIP-1a, and MIP-2 in BV-2 microglial cells; these increases were initiated via MyD88-dependent TLR-2/TLR-4. The production levels of proinflammatory cytokines and chemokines in NfEVs-stimulated BV-2 microglial cells were effectively downregulated by inhibitors of MAPK, NF-?B, or JAK-STAT. Phosphorylation levels of JNK, p38, ERK, p65, JAK-1, and STAT3 were increased in NfEVs-stimulated BV-2 microglial cells but were effectively suppressed by each corresponding inhibitor. These results suggest that NfEVs could induce proinflammatory immune responses in BV-2 microglial cells via the NF-?B-dependent MAPK and JAK-STAT signaling pathways. Taken together, these findings suggest that NfEVs are pathogenic factors involved in the contact-independent pathogenic mechanisms of N. fowleri by inducing proinflammatory immune responses in BV-2 microglial cells, further contributing to deleterious inflammation in infected foci by activating subsequent inflammation cascades in other brain cells. | - |
dc.language | 영어 | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Naegleria fowleri Extracellular Vesicles Induce Proinflammatory Immune Responses in BV-2 Microglial Cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/ijms241713623 | - |
dc.citation.journaltitle | International Journal of Molecular Sciences | - |
dc.identifier.wosid | 001064188500001 | - |
dc.identifier.scopusid | 2-s2.0-85170254817 | - |
dc.citation.number | 17 | - |
dc.citation.startpage | 13623 | - |
dc.citation.volume | 24 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Yoo, Won Gi | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSES | - |
dc.subject.keywordPlus | COMMUNICATION | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordAuthor | Naegleria fowleri | - |
dc.subject.keywordAuthor | extracellular vesicles | - |
dc.subject.keywordAuthor | microglial cells | - |
dc.subject.keywordAuthor | proinflammatory response | - |
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