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Intratesticular Peptidyl Prolyl Isomerase 1 Protein Delivery Using Cationic Lipid-Coated Fibroin Nanoparticle Complexes Rescues Male Infertility in Mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Woo Jin | - |
dc.contributor.author | Kim, Bong Soo | - |
dc.contributor.author | Kim, Hyun Jung | - |
dc.contributor.author | Cho, Young Dan | - |
dc.contributor.author | Shin, Hye Lim | - |
dc.contributor.author | Yoon, Hee In | - |
dc.contributor.author | Lee, Yun Sil | - |
dc.contributor.author | Baek, Jeong-Hwa | - |
dc.contributor.author | Woo, Kyung Mi | - |
dc.contributor.author | Ryoo, Hyun-Mo | - |
dc.date.accessioned | 2024-05-02T05:58:23Z | - |
dc.date.available | 2024-05-02T05:58:23Z | - |
dc.date.created | 2021-02-26 | - |
dc.date.created | 2021-02-26 | - |
dc.date.created | 2021-02-26 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | ACS Nano, Vol.14 No.10, pp.13217-13231 | - |
dc.identifier.issn | 1936-0851 | - |
dc.identifier.uri | https://hdl.handle.net/10371/200485 | - |
dc.description.abstract | Male infertility is a multifactorial condition. Unexplained male infertility is often caused by spermatogenesis dysfunction. Knockout of Pin1, an important regulator of cell proliferation and differentiation, produces male infertility phenotypes such as testicular immaturity and azoospermia with spermatogonia depletion and blood-testis barrier (BTB) dysfunction. Gene therapy has been clinically considered for the treatment of male infertility, but it is not preferred because of the risks of adverse effects in germ cells. Direct intracellular protein delivery using nanoparticles is considered an effective alternative to gene therapy; however, in vivo testicular protein delivery remains a pressing challenge. Here, we investigated the direct intracellular protein delivery strategy using a fibroin nanoparticle-encapsulated cationic lipid complex (Fibroplex) to restore intratesticular PIN1. Local intratesticular delivery of PIN1 via Fibroplex in Pin1 knockout testes produced fertile mice, achieving recovery from the infertile phenotypes. Mechanistically, PIN1-loaded Fibroplex was successfully delivered into testicular cells, including spermatogonial cells and Sertoli cells, and the sustained release of PIN1 restored the gene expression required for the proliferation of spermatogonial cells and BTB integrity in Pin1 knockout testes. Collectively, testicular PIN1 protein delivery using Fibroplex might be an effective strategy for treating male infertility. | - |
dc.language | 영어 | - |
dc.publisher | American Chemical Society | - |
dc.title | Intratesticular Peptidyl Prolyl Isomerase 1 Protein Delivery Using Cationic Lipid-Coated Fibroin Nanoparticle Complexes Rescues Male Infertility in Mice | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acsnano.0c04936 | - |
dc.citation.journaltitle | ACS Nano | - |
dc.identifier.wosid | 000586793400074 | - |
dc.identifier.scopusid | 2-s2.0-85094983184 | - |
dc.citation.endpage | 13231 | - |
dc.citation.number | 10 | - |
dc.citation.startpage | 13217 | - |
dc.citation.volume | 14 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Cho, Young Dan | - |
dc.contributor.affiliatedAuthor | Lee, Yun Sil | - |
dc.contributor.affiliatedAuthor | Baek, Jeong-Hwa | - |
dc.contributor.affiliatedAuthor | Woo, Kyung Mi | - |
dc.contributor.affiliatedAuthor | Ryoo, Hyun-Mo | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BLOOD-TESTIS BARRIER | - |
dc.subject.keywordPlus | SPERMATOGONIAL STEM-CELL | - |
dc.subject.keywordPlus | ANDROGEN RECEPTOR | - |
dc.subject.keywordPlus | PIN1 | - |
dc.subject.keywordPlus | SERTOLI | - |
dc.subject.keywordPlus | SPERMATOGENESIS | - |
dc.subject.keywordPlus | SPERM | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | AZOOSPERMIA | - |
dc.subject.keywordAuthor | intracellular protein delivery | - |
dc.subject.keywordAuthor | cationic lipid-coated fibroin nanoparttcle complex | - |
dc.subject.keywordAuthor | Pinl | - |
dc.subject.keywordAuthor | male infertility | - |
dc.subject.keywordAuthor | protein therapeutics | - |
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