Publications
Detailed Information
Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-κB-dependent MAPK pathways
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Jung-Mi | - |
dc.contributor.author | Yoo, Won Gi | - |
dc.contributor.author | Le, Huong Giang | - |
dc.contributor.author | Lee, Jinyoung | - |
dc.contributor.author | Sohn, Woon-Mok | - |
dc.contributor.author | Na, Byoung-Kuk | - |
dc.date.accessioned | 2024-05-02T05:59:26Z | - |
dc.date.available | 2024-05-02T05:59:26Z | - |
dc.date.created | 2024-04-29 | - |
dc.date.created | 2024-04-29 | - |
dc.date.created | 2024-04-29 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.citation | Parasites and Vectors, Vol.13 No.1, p. 20 | - |
dc.identifier.issn | 1756-3305 | - |
dc.identifier.uri | https://hdl.handle.net/10371/200504 | - |
dc.description.abstract | Background MF6p/host defense molecules (HDMs) are a broad family of small proteins secreted by helminth parasites. Although the physiological role of MF6p/HDMs in trematode parasites is not fully understood, their potential biological function in maintaining heme homeostasis and modulating host immune response has been proposed. Methods A gene encoding the MF6p/HDM of Clonorchis sinensis (CsMF6p/HDM) was cloned. Recombinant CsMF6p/HDM (rCsMF6p/HDM) was expressed in Escherichia coli. The biochemical and immunological properties of rCsMF6/HDM were analyzed. CsMF6p/HDM induced pro-inflammatory response in RAW 264.7 cells was analyzed by cytokine array assay, reverse transcription polymerase chain reaction, and enzyme-linked immunosorbent assay. The structural feature of CsMF6p/HDM was analyzed by three-dimensional modeling and molecular docking simulations. Results The CsMF6p/HDM shares a high level of amino acid sequence similarity with orthologs from other trematodes and is expressed in diverse developmental stages of the parasite. The rCsMF6p/HDM bound to bacteria-derived lipopolysaccharide (LPS), without effectively neutralizing LPS-induced inflammatory response in RAW 264.7 macrophage cells. Rather, the rCsMF6p/HDM induced pro-inflammatory immune response, which is characterized by the expression of TNF-alpha and IL-6, in RAW 264.7 cells. The rCsMF6p/HDM-induced pro-inflammatory immune response was regulated by JNK and p38 MAPKs, and was effectively down-regulated via inhibition of NF-kappa B. The structural analysis of CsMF6p/HDM and the docking simulation with LPS suggested insufficient capture of LPS by CsMF6p/HDM, which suggested that rCsMF6p/HDM could not effectively neutralize LPS-induced inflammatory response in RAW 264.7 cells. Conclusions Although rCsMF6p/HDM binds to LPS, the binding affinity may not be sufficient to maintain a stable complex of rCsMF6p/HDM and LPS. Moreover, the rCsMF6p/HDM-induced pro-inflammatory response is characterized by the release of IL-6 and TNF-alpha in RAW 264.7 macrophage cells. The pro-inflammatory response induced by rCsMF6p/HDM is mediated via NF-kappa B-dependent MAPK signaling pathway. These results collectively suggest that CsMF6p/HDM mediates C. sinensis-induced inflammation cascades that eventually lead to hepatobiliary diseases. | - |
dc.language | 영어 | - |
dc.publisher | BioMed Central | - |
dc.title | Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-κB-dependent MAPK pathways | - |
dc.type | Article | - |
dc.identifier.doi | 10.1186/s13071-020-3882-0 | - |
dc.citation.journaltitle | Parasites and Vectors | - |
dc.identifier.wosid | 000513780600001 | - |
dc.identifier.scopusid | 2-s2.0-85077785761 | - |
dc.citation.number | 1 | - |
dc.citation.startpage | 20 | - |
dc.citation.volume | 13 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Yoo, Won Gi | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PROTEIN-STRUCTURE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | ANTIGEN | - |
dc.subject.keywordPlus | SERVER | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | HEME | - |
dc.subject.keywordAuthor | Clonorchis sinensis | - |
dc.subject.keywordAuthor | MF6p | - |
dc.subject.keywordAuthor | host defense molecule | - |
dc.subject.keywordAuthor | Lipopolysaccharide | - |
dc.subject.keywordAuthor | Pro-inflammatory immune response | - |
dc.subject.keywordAuthor | Structure | - |
dc.subject.keywordAuthor | Docking | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.