Publications

Detailed Information

Fibrous topography-potentiated canonical Wnt signaling directs the odontoblastic differentiation of dental pulp-derived stem cells

Cited 39 time in Web of Science Cited 41 time in Scopus
Authors

Rahman, Saeed Ur; Oh, Joung-Hwan; Cho, Young-Dan; Chung, Shin Hye; Lee, Gene; Baek, Jeong-Hwa; Ryoo, Hyun-Mo; Woo, Kyung Mi

Issue Date
2018-05
Publisher
American Chemical Society
Citation
ACS Applied Materials and Interfaces, Vol.10 No.21, pp.17526-17541
Abstract
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, the mechanisms by which the nanofibrous matrix promotes mesenchymal stem cell (MSC) differentiation are largely unknown. Here, we investigated the mechanisms that underlie nanofibrous matrix-induced odontoblastic differentiation of human dental pulp MSCs (DP-MSCs). An electrospun polystyrene nanofibrous (PSF) matrix was prepared, and the cell responses to the PSF matrix were assessed in comparison with those on conventional tissue culture dishes. The PSF matrix promoted the expression of Wnt3a, Wnt5a, Wnt10a, BMP2, BMP4, and BMP7 in the DP-MSCs, concomitant with the induction of odontoblast/osteoblast differentiation markers, dentin sialophosphoprotein (DSPP), osteocalcin, and bone sialoprotein, whose levels were further enhanced by treatment with recombinant Wnt3a. The DP-MSCs cultured on the PSF matrix also exhibited a high alkaline phosphatase activity and intense Alizarin Red staining, indicating that the PSF matrix promotes odontoblast differentiation. Besides inducing the expression of Wnt3a, the PSF matrix maintained high levels of beta-catenin protein and enhanced its translocation to the nucleus, leading to its transcriptional activity. Forced expression of LEF1 or treatments with LiC1 further enhanced the DSPP expression. Blocking the Wnt3a-initiated signaling abrogated the PSF-induced DSPP expression. Furthermore, the cells on the PSF matrix increased the DSPP promoter activity. The beta-catenin complex was bound to the conserved motifs on the DSPP promoter dictating its transcription. Transplantations of the preodontoblast-seeded PSF matrix to the subcutaneous tissues of nude mice confirmed the association of the PSF matrix with the Wnt3a and DSPP expressions in vivo. Taken together, these results demonstrate the nanofibrous engineered matrix strongly supports odontoblastic differentiation of DP-MSCs by enhancing Wnt/beta-catenin signaling.
ISSN
1944-8244
URI
https://hdl.handle.net/10371/200560
DOI
https://doi.org/10.1021/acsami.7b19782
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Related Researcher

Cho, Young-Dan Image

Cho, Young-Dan조영단
(기금)조교수
  • School of Dentistry
  • Department of Dentistry
Research Area Alveolar bone regeneration, Dental implant surface modification, Periomics

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share