Publications
Detailed Information
TOC1 in Nicotiana attenuata regulates efficient allocation of nitrogen to defense metabolites under herbivory stress : <i>TOC1</i>in<i>Nicotiana attenuata</i>regulates efficient allocation of nitrogen to defense metabolites under herbivory stress
Cited 8 time in
Web of Science
Cited 11 time in Scopus
- Authors
- Issue Date
- 2020-11
- Publisher
- WILEY
- Citation
- NEW PHYTOLOGIST, Vol.228 No.4, pp.1227-1242
- Abstract
- The circadian clock contextualizes plant responses to environmental signals. Plants use temporal information to respond to herbivory, but many of the functional roles of circadian clock components in these responses, and their contribution to fitness, remain unknown. We investigate the role of the central clock regulator TIMING OF CAB EXPRESSION 1 (TOC1) inNicotiana attenuata's defense responses to the specialist herbivoreManduca sextaunder both field and glasshouse conditions. We utilize(15)N pulse-labeling to quantify nitrogen incorporation into pools of three defense compounds: caffeoylputrescine (CP), dicaffeoyl spermidine (DCS) and nicotine. Nitrogen incorporation was decreased in CP and DCS and increased in nicotine pools in irTOC1 plants compared to empty vector (EV) under control conditions, but these differences were abolished after simulated herbivory. Differences between EV and irTOC1 plants in nicotine, but not phenolamide production, were abolished by treatment with the ethylene agonist 1-methylcyclopropene. Using micrografting,TOC1's effect on nicotine was isolated to the root and did not affect the fitness of heterografts under field conditions. These results suggest that the circadian clock contributes to plant fitness by balancing production of metabolically expensive nitrogen-rich defense compounds and mediating the allocation of resources between vegetative biomass and reproduction.
- ISSN
- 0028-646X
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.