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Comparative cost and performance of light-emitting diode microscopy in HIV-tuberculosis-co-infected patients
DC Field | Value | Language |
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dc.contributor.author | Whitelaw, A. | - |
dc.contributor.author | Peter, J. | - |
dc.contributor.author | Sohn, H. | - |
dc.contributor.author | Viljoen, D. | - |
dc.contributor.author | Theron, G. | - |
dc.contributor.author | Badri, M. | - |
dc.contributor.author | Davids, V. | - |
dc.contributor.author | Pai, M. | - |
dc.contributor.author | Dheda, K. | - |
dc.date.accessioned | 2024-05-14T04:35:48Z | - |
dc.date.available | 2024-05-14T04:35:48Z | - |
dc.date.created | 2024-05-14 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | EUROPEAN RESPIRATORY JOURNAL, Vol.38 No.6, pp.1393-1397 | - |
dc.identifier.issn | 0903-1936 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201773 | - |
dc.description.abstract | Light-emitting diode (LED) microscopy has recently been endorsed by the World Health Organization (WHO). However, it is unclear whether LED is as accurate and cost-effective as Ziehl-Neelsen (ZN) microscopy or mercury vapour fluorescence microscopy (MVFM) in tuberculosis (TB)-HIV-co-infected subjects. Direct and concentrated sputum smears from TB suspects were evaluated using combinations of LED microscopy, ZN microscopy and MVFM. Median reading time per slide was recorded and a cost analysis performed. Mycobacterial culture served as the reference standard. 647 sputum samples were obtained from 354 patients (88 (29.8%) were HIV-infected and 161 (26%) were culture-positive for Mycobacterium tuberculosis). Although overall sensitivity of LED compared with ZN microscopy or MVFM was similar, sensitivity of all three modalities was lower in HIV-infected patients. In the HIV-infected group, the sensitivity of LED microscopy was higher than ZN microscopy using samples that were not concentrated (46 versus 39%; p=0.25), and better than MVFM using concentrated samples (56 versus 44; p=0.5). A similar trend was seen in the CD4 count <200 cells.mL(-1) subgroup. Median (interquartile range) reading time was quicker with LED compared with ZN microscopy (1.8 (1.7-1.9) versus 2.5 (2.2-2.7) min; p <= 0.001). Average cost per slide read was less for LED microscopy (US$1.63) compared with ZN microscopy (US$2.10). Among HIV-TB-co-infected patients, LED microscopy was cheaper and performed as well as ZN microscopy or MVFM independent of the staining (ZN or auramine O) or processing methods used. | - |
dc.language | 영어 | - |
dc.publisher | EUROPEAN RESPIRATORY SOC JOURNALS LTD | - |
dc.title | Comparative cost and performance of light-emitting diode microscopy in HIV-tuberculosis-co-infected patients | - |
dc.type | Article | - |
dc.identifier.doi | 10.1183/09031936.00023211 | - |
dc.citation.journaltitle | EUROPEAN RESPIRATORY JOURNAL | - |
dc.identifier.wosid | 000298155600024 | - |
dc.identifier.scopusid | 2-s2.0-82555194430 | - |
dc.citation.endpage | 1397 | - |
dc.citation.number | 6 | - |
dc.citation.startpage | 1393 | - |
dc.citation.volume | 38 | - |
dc.description.isOpenAccess | Y | - |
dc.contributor.affiliatedAuthor | Sohn, H. | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | MYCOBACTERIUM-TUBERCULOSIS | - |
dc.subject.keywordPlus | FLUORESCENCE MICROSCOPY | - |
dc.subject.keywordPlus | ZIEHL-NEELSEN | - |
dc.subject.keywordAuthor | HIV | - |
dc.subject.keywordAuthor | light-emitting diode microscopy | - |
dc.subject.keywordAuthor | smear microscopy | - |
dc.subject.keywordAuthor | tuberculosis | - |
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