Publications
Detailed Information
Time-resolved profiling of RNA binding proteins throughout the mRNA life cycle
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Yeon | - |
dc.contributor.author | Um, Buyeon | - |
dc.contributor.author | Na, Yongwoo | - |
dc.contributor.author | Kim, Jeesoo | - |
dc.contributor.author | Kim, Jong-Seo | - |
dc.contributor.author | Kim, V. Narry | - |
dc.date.accessioned | 2024-05-14T06:47:32Z | - |
dc.date.available | 2024-05-14T06:47:32Z | - |
dc.date.created | 2024-05-14 | - |
dc.date.created | 2024-05-14 | - |
dc.date.issued | 2024-05 | - |
dc.identifier.citation | Molecular Cell, Vol.84 No.9, pp.1764-1782.e10 | - |
dc.identifier.issn | 1097-2765 | - |
dc.identifier.uri | https://hdl.handle.net/10371/201870 | - |
dc.description.abstract | mRNAs continually change their protein partners throughout their lifetimes, yet our understanding of mRNA-protein complex (mRNP) remodeling is limited by a lack of temporal data. Here, we present time-resolved mRNA interactome data by performing pulse metabolic labeling with photoactivatable ribonucleoside in human cells, UVA crosslinking, poly(A)+ RNA isolation, and mass spectrometry. This longitudinal approach allowed the quantification of over 700 RNA binding proteins (RBPs) across ten time points. Overall, the sequential order of mRNA binding aligns well with known functions, subcellular locations, and molecular interactions. However, we also observed RBPs with unexpected dynamics: the transcription-export (TREX) complex recruited posttranscriptionally after nuclear export factor 1 (NXF1) binding, challenging the current view of transcription-coupled mRNA export, and stress granule proteins prevalent in aged mRNPs, indicating roles in late stages of the mRNA life cycle. To systematically identify mRBPs with unknown functions, we employed machine learning to compare mRNA binding dynamics with Gene Ontology (GO) annotations. Our data can be explored at chronology.rna.snu.ac.kr. | - |
dc.language | 영어 | - |
dc.publisher | Cell Press | - |
dc.title | Time-resolved profiling of RNA binding proteins throughout the mRNA life cycle | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.molcel.2024.03.012 | - |
dc.citation.journaltitle | Molecular Cell | - |
dc.identifier.wosid | 001238537900001 | - |
dc.identifier.scopusid | 2-s2.0-85191331694 | - |
dc.citation.endpage | 1782.e10 | - |
dc.citation.number | 9 | - |
dc.citation.startpage | 1764 | - |
dc.citation.volume | 84 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Kim, Jong-Seo | - |
dc.contributor.affiliatedAuthor | Kim, V. Narry | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | TRANSLATIONAL CONTROL | - |
dc.subject.keywordPlus | STRESS GRANULES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | NUCLEAR | - |
dc.subject.keywordPlus | DISCOVERY | - |
dc.subject.keywordPlus | PROTEOME | - |
dc.subject.keywordPlus | GENOME | - |
dc.subject.keywordPlus | EXPORT | - |
dc.subject.keywordPlus | SITES | - |
dc.subject.keywordPlus | NXF1 | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordAuthor | mRNA binding dynamics | - |
dc.subject.keywordAuthor | mRNA life-cycle | - |
dc.subject.keywordAuthor | mRNA nuclear export | - |
dc.subject.keywordAuthor | NXF1 | - |
dc.subject.keywordAuthor | pulse-chase labeling | - |
dc.subject.keywordAuthor | RNA binding protein | - |
dc.subject.keywordAuthor | RNA interactome capture | - |
dc.subject.keywordAuthor | stress granule | - |
dc.subject.keywordAuthor | TREX complex | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.